@article { author = {Yaripour, Sareh and Esmaeili, Hamid Reza and Gholamhosseini, Ali and Rezaei, Mohammad and Sadeghi, Saber}, title = {Assessment of genetic diversity of an endangered tooth-carp, Aphanius farsicus (Teleostei: Cyprinodontiformes: Cyprinodontidae) using microsatellite markers}, journal = {Molecular Biology Research Communications}, volume = {6}, number = {4}, pages = {153-160}, year = {2017}, publisher = {Shiraz University Press}, issn = {2322-181X}, eissn = {2345-2005}, doi = {10.22099/mbrc.2017.24404.1246}, abstract = {Genetic structure of an endemic tooth-carp fish, Aphanius farsicus from four different water bodies in the Maharlu Lake basin was investigated by applying five microsatellite markers. All of the five examined microsatellite loci showed polymor-phism pattern. A total of four alleles were detected at five microsatellite loci, with an average of 2.8 to 3.5 alleles per locus. Average values of observed and expected heterozygosity were 0.95±0.09 and 0.64±0.02 respectively. None of the tests of linkage disequilibrium were significant between each pair of loci and no deviation from Hardy-Weinberg equilibrium were detected to test for heterozygote deficiency within populations. The Nei's genetic distance values ranged between 0.03 – 0.13. Analysis of pairwise genetic differentiation between each pair of the populations revealed that fixation index (FST) values ranged from 0.013 to 0.039 and RST ranged from 0.005 to 0.065. High genetic diversity observed within the populations (99%) and low diversity (1%) among them indicating probably high level of gene flow among the studied populations of Fars tooth-carp at the present time or in the past. Regarding low genetic differentiation among the studied populations and results of population assignment test, two hypotheses are suggested and supporting evidence for each hypothesis are provided.}, keywords = {Maharlu Lake,Genetic differentiation,Microsatellite,Tooth-carp fishes}, url = {https://mbrc.shirazu.ac.ir/article_4191.html}, eprint = {https://mbrc.shirazu.ac.ir/article_4191_8f833d53c596af8d609b7107f40469fe.pdf} } @article { author = {Eraghi, Vida and Derakhshandeh, Abdollah and Hosseini, Arsalan and Motamedi-Boroojeni, Azar}, title = {In silico design and expression of a novel fusion protein of HBHA and high antigenic region of FAP-P of Mycobacterium avium subsp. paratuberculosis in Pichia pastoris}, journal = {Molecular Biology Research Communications}, volume = {6}, number = {4}, pages = {161-168}, year = {2017}, publisher = {Shiraz University Press}, issn = {2322-181X}, eissn = {2345-2005}, doi = {10.22099/mbrc.2017.26522.1286}, abstract = {Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in ruminants and there has been a shift in the public health approach to MAP and human diseases like Crohn's disease. The prevention of infection by MAP in ruminants is thought to deter the high impact of economic losses in the level of dairy industry and possible spreading of this pathogen in dairy products. The present study was done to investigate the construction and expression of the soluble form of a novel fusion protein, consisting of Heparin-binding hemagglutinin (HBHA) and high antigenic region of Fibronectin Attachment Protein-P (FAP-P), in order to introduce as a Th1 inducer subunit vaccine against MAP. HBHA is a mycobacterial adhesin and it has been demonstrated that a HBHA-specific IFN-γ response, in latent M. tuberculosis infection, depends on the methylation of the antigen. Further, FAP-P induces Th1 polarization. Because methylation of HBHA was not performed in E. coli, Pichia pastoris was chosen as the host. The desired fusion protein had a similar 3D structure to that of HBHA with its native form and post-translational methylation in C-terminal. Hence, the uptake of the purified fusion protein will be done by M cells because ofHBHA, and cell-mediated immunity will be induced because of both antigens. Eventually, successful construction and expression of the newly-designed chimeric protein under the mentioned conditions is reported in this article.}, keywords = {FAP-P,fusion protein,HBHA,In silico,Mycobacterium avium subsp}, url = {https://mbrc.shirazu.ac.ir/article_4343.html}, eprint = {https://mbrc.shirazu.ac.ir/article_4343_007a34924514ef8b4215a32094cf6b00.pdf} } @article { author = {Shakeran, Zahra and Keyhanfar, Mehrnaz and Ghanadian, Mustafa}, title = {Biotic elicitation for scopolamine production by hairy root cultures of Datura metel}, journal = {Molecular Biology Research Communications}, volume = {6}, number = {4}, pages = {169-176}, year = {2017}, publisher = {Shiraz University Press}, issn = {2322-181X}, eissn = {2345-2005}, doi = {10.22099/mbrc.2017.25776.1275}, abstract = {The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was used as an advantageous method for production of scopolamine. The hairy roots are formed by Agrobacterium rhizogenes and have genetic stability, high growth rate and lateral branching. In this study, the effect of Bacillus cereus and Staphylococcus aureus as biotic elicitors on the production of scopolamine in D. metel hairy roots was investigated. The amount of scopolamine in the hairy roots was detected by HPLC analysis and compared with control samples after 0, 12 and 24 hours. Results showed that, B. cereus and S. aureus enhanced scopolamine production in the culture while the atropine content was decreased. Although in the control samples with no bacterial elicitation no scopolamine was detected, elicitation by B. cereus caused production of scopolamine and about 0.03 gram and 0.017 gram of it was detected in 100 gram dried D. metel hairy roots after 12 and 24 hours respectively.  In S. aureus elicited hairy roots, scopolamine was not produced after 12 hours. However, about 0.025 gram of this tropane alkaloid was detected in 100 gram dried hairy roots after 24 hours. In conclusion, S. areus and B. cereus induced the scopolamine production in D. metel hairy roots.}, keywords = {Secondary metabolites,Bacillus cereus,Staphylococcus aureus}, url = {https://mbrc.shirazu.ac.ir/article_4345.html}, eprint = {https://mbrc.shirazu.ac.ir/article_4345_870f52ce97e0548f589551b0b1ed2122.pdf} } @article { author = {Safaie-Farahani, Ali and Taghavi, S.Mohsen}, title = {Transcript analysis of some defense genes of tomato in response to host and non-host bacterial pathogens}, journal = {Molecular Biology Research Communications}, volume = {6}, number = {4}, pages = {177-183}, year = {2017}, publisher = {Shiraz University Press}, issn = {2322-181X}, eissn = {2345-2005}, doi = {10.22099/mbrc.2017.25600.1273}, abstract = {The transcript levels of six defense genes including pathogenesis-related gene 1 (PR-1), pathogenesis-related gene 2 (PR-2), pathogenesis-related gene 5 (PR-5), lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and catalase (CAT) were investigated in tomato plants inoculated with Xanthomonas axonopodis pv. phaseoli as a non-host pathogen and X. euvesicatoria as a host pathogen. Activation of all the genes was confirmed in both host and non-host treatments. Additionally, the results showed stronger expression of majority of the genes (PR-1, PR-2, LOX, PAL and CAT) in non-host treatment compared to host treatment at least at early hours after inoculation. These data suggest that faster and more expression of PR-1, PR-2, LOX, PAL and CAT might have a role in non-host resistance of tomato against X. axonopodis pv. phaseoli.}, keywords = {PR-1,PR-2,PR-5,LOX,PAL,Cat}, url = {https://mbrc.shirazu.ac.ir/article_4346.html}, eprint = {https://mbrc.shirazu.ac.ir/article_4346_ac36c4527e3f0a29020abc950afd071b.pdf} }