TY - JOUR ID - 2517 TI - Karyological and molecular analysis of three endemic loaches (Actinopterygii: Cobitoidea) from Kor River basin, Iran JO - Molecular Biology Research Communications JA - MBRC LA - en SN - 2322-181X AU - Esmaeili, Hamid Reza AU - Piravar, Zeinab AU - Ebrahimi, Mehrgan AU - Geiger, Matthias F AD - Department of Biology, College of Sciences, Shiraz University AD - Shiraz University AD - Zoological Research Museum Alexander Koenig, Leibniz Institute for Animal Biodiversity, Y1 - 2015 PY - 2015 VL - 4 IS - 1 SP - 1 EP - 13 KW - Loaches KW - Phylogenetic relationships KW - COI barcode region KW - idiogram KW - Iran DO - 10.22099/mbrc.2015.2517 N2 - This study provides new data on chromosomal characteristics and DNA barcoding of three endemic loaches of Iran: spiny southern loach Cobitis linea (Heckel, 1847), Persian stream loach Oxynoemacheilus persa (Heckel, 1848) and Tongiorgi stream loach Oxynoemacheilus tongiorgii (Nalbant & Bianco, 1998). The chromosomes of these fishes were investigated by examining metaphase chromosome spreads obtained from epithelial gill and kidney cells. The diploid chromosome numbers of all three species were 2n=50. The karyotypes of C. linea consisted of 4M + 40SM + 6ST, NF=94; of O. persa by 20M + 22SM + 8ST, NF=90 and of O. tongiorgii by 18M + 24SM + 8ST, NF= 92. Sex chromosomes were cytologically indistinguishable in these loaches. Maximum likelihood-based estimation of the phylogenetic relationships based on the COI barcode region clearly separates the three Iranian loach species of the Kor River basin. All species distinguished by morphological characters were recovered as monophyletic clades by the COI barcodes. The obtained results could be used for population studies, management and conservation programs. UR - https://mbrc.shirazu.ac.ir/article_2517.html L1 - https://mbrc.shirazu.ac.ir/article_2517_c633dda0f46deb28d86da57ca3f39684.pdf ER -