1Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran
2Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
3Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Malonyl-CoA decarboxylase (MCD) is an enzyme involved in the decarboxylation of malonyl-CoA to acetyl-CoA. In order to explore the hypothesis that the changing plant materials’ MCD activity level can serve as therapy to diabetics, the effect of Teucrium polium compounds was studied in a diabetic rat model. In this experimental study, two groups of rats, a control and a diabetic group, each including six rats, were used. At the end of the experiment, all rats were exterminated by ether anesthesia, their pancreases removed and dissected. Isolated rat pancreas was cultured in buffers with or without 100-500µg/l T. polium aerial parts extracts containing arginine and leucine. MCD and insulin levels were measured after culture at 37°C and 5% CO2, for 1, 3 and 5 days. Results showed that T. polium aqueous and the alcoholic extract decreased MCD activity. Present data also indicate that incubation of pancreatic tissue at a concentration of 2.8 and 16.7 mmol/L glucose stimulated insulin release. For the first time it seems that aqueous and alcoholic extracts of this plant decreased MCD activity.
3.Afifi FU, Al-Khalidi B, Khalil E. Studies on the in vivo hypoglycemic activities of two medicinal plants used in the treatment of diabetes in Jordanian traditional medicine following intranasal administration.J Ethnopharmacol 2005;100:314-318.
4.Iriadam M, Musa D, Gumushan H, Baba F. Effects of two Turkish medicinal plants Artemisia herba-albaand Teucrium polium on blood glucose levels and other biochemical parameters in rabbits.J Cell Mol Biol 2006;5:19-24.
5.Shahraki MR, Arab MR, Mirimokaddam E, Palan MJ. The effect of Teucrium polium (Calpoureh) on liver function, serum lipids and glucose in diabetic male rats. Iran Biomed J 2007;11:65-68.
6.Esmaeili MA, Yazdanparast R. Hypoglycaemic effect of Teucrium polium: studies with rat pancreatic islets. J Ethnopharmacol 2004;95:27-30.
7.Habinowski SA, Hirshman M, Sakamoto K, Kemp BE, Gould SJ, Goodyear LJ and Witters LA. Malonyl-CoA decarboxylase is not a substrate of AMP-activated protein kinase in rat fast-twitch skeletal muscle or islet cell line. Arch Biochem Biophys 2001;396:71-79.
8.McGarry JD, Woeltje KF, Kuwajima M, Foster DW. Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase. Diabetes Metab 1989;5:271-284.
9.Bandyopadhyay GK, Yu G, Ofrecio J, Olefsky JM. Increased Malonyl-CoA levels in muscle from obese and type 2 diabetic subjects lead to decreased fatty acid oxidation and increased lipogenesis; thiazolidinedione treatment reverses these defects. Diabetes 2006;55:2277-2285.
10.Tang H, Yan Y, Feng Z, Jesus RK, Yang L, Levorse DA. Design and synthesis of a new class of malonyl-CoA decarboxylase inhibitors with anti-obesity and anti-diabetic activities. Bioorg Med Chem Lett 2010;20:6088-6092.
11.Folmes CDL, Lopaschuk GD. Role of malonyl-CoA in heart disease and the hypothalamic control of obesity. Cardiovascular Res 2007;73:278-287.
12.Buckner JS, Kolattukudy PE, Poulose AJ. Purification and properties of malonyl-coenzyme A decarboxylase, a regulatory enzyme from the uropygial gland of goose. Arch Biochem Biophys 1976;177:539-551.
13.Ruderman NB, Asish KS. Metabolic syndrome: adenosine monophosphate-activated protein kinase and malonyl coenzyme A. Obesity 2006;14:25-33.