Phylogenetic analysis of Persian Gazella, Gazella subgutturosa (Artiodactyla: Bovidae) based on cytochrome b in central Iran

Document Type: Original article


1 Environmental Sciences Department, Gorgan University of Agriculture and Natural Resources

2 Environmental Sciences Department, Gorgan University of Agriculture and Natural Resources, Gorgan, Iran.

3 Natural Resources Faculty, University of Guilan, Guilan, Iran

4 Laboratoire d’Ecologie Alpine, CNRS UMR 5553, Université Joseph Fourier, B.P. 53, F-38041 Grenoble Cedex 9, France.


Persian gazelle, Gazella subgutturosa, exists throughout arid and semiarid regions of Iran and has a key role in these frail ecosystems. Habitat degradation and population decline has placed it on the list of vulnerable species in 2008. The phylogenetic relationships of three Persian gazelle populations in the central part of Iran (i.e. Ghamishlou National Park and Wildlife Refuge, Mouteh Wildlife Refuge in Isfahan province and Kalmand-Bahadoran Protected Area in Yazd province) were investigated using parts and short fragments of mitochondrial cytochrome b gene (425 base pairs). A maximum likelihood phylogenetic tree separated the populations of Yazd and Isfahan provinces, but populations within the Isfahan province shared the same clade. All populations were classified as Persian gazelle. The studied populations are facing threats because of road construction, industrial development and urbanization. Accordingly urgent conservation plans are needed to preserve their genetic diversity and prevent them from falling into extinction.


1.Karami M, Hemami MR, Groves CP. Taxonomic, distribution and ecological data on gazelles in Iran. Zool Middle East 2002;26:29-36.

2.Zachos FE, Karami M, Ibenouazi Z, Hartl GB, Eckert I, Kirschning J. First genetic analysis of afree-living population of the threatened goitered gazelle (Gazella subgutturosa). Mamm Biol 2009;75:277-282.

3.Ziaie H. A field guide to the mammals of Iran. Iran Wild life Center, Tehran, Iran. 2008;432. [In Persian]

4.Firouz E. The complete fauna of Iran. I. B. Tauris, London, New York. 2005;250.

5.IUCN. IUCN Red List of threatened Species. IUCN, Gland, Switzerland and Cambridge, UK, 2010.

6.Lerp H, Wronski T, Pfenninger M, Plath M. A phylogeographic framework for the conservation of Saharan and Arabian Dorcas gazelles (Artiodactyla: Bovidae). Org Divers Evol 2011;11:317-329.

7.Mallon DP, Kingswood SP. Antelopes. Part IV. North Africa, the Middle East and Asia. Global survey and regional action plans. SSC Antelope Specialist Group, IUCN, Cambridge. 2001;260.

8.Wacher T, Wronski T, Hammond RL, Winney B, Blacket MJ, Hundertmark KJ, Mohammed OB, Ome SA, Macasero W, Lerp H, Plath M, Bleidorn C. Phylogenetic analysis of mitochondrial DNA sequences reveals polyphyly in the Goitred gazelle (Gazella subgutturosa). Conserv Genet 2011;12:827-831.

9.Hammond R L, Macasero W, Flores B, Mohammed OB, Wacher TW, Bruford MW. Phylogenetic re-analysis of the Saudi gazelle and its implications for conservation. Conserv Biol 2001;15:1123-1133.

10.Irwin DL, Kocher TD, Wilson AC. The evolution of cytochrome b gene in mammals. J Mol Evol 1991;32:128-144.

11.Akbari H, Behrouzi rad B, Kiabi BH. Habitat evaluation of Persian gazelle in Kalmand Protected Area. Environ J 2002;46:113-118. [In Persian]

12.Farhadinia MS, Esfandabad BS, Karami M, Zavarei FH, Absalan H, Nezami B. Goitered Gazelle Gazelle subgutturosa Guldenstaedt, 1780: its habitat preference and conservation needs in Miandasht Wildlife Refuge, northeastern Iran. Zool Middle East 2009;46:9-18.

13.Freeland J. Molecular ecology. John Wiley & Sons Ltd, the Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England. 2005; 388P.

14.Avise, JC. A role for molecular genetics in the recognition and conservation of endangered species. Trends Ecol Evol 1989;4:279-281.

15.Vogler AP, DeSalle R. Diagnosing units of conservation management. Conserv Biol 1994;8:354–363.

16.Ryder OA. Conservation action for Gazelles - an urgent need. Trends Ecol Evol 1987;6:143-144.

17.Kocher TD, Thomas WK, Meyer A, Edwards SV, Paabo S, Villablanca FX, Wilson AC. Dynamics of mitochondrial evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci USA 1989;86:6196-6200.

18.Rebholz WER, Harley EH. Cytochrome b Sequences from the Endangered Saudi Gazelle (Gazella saudiya) suggest hybridization with chinkara (G. bennetti). Conserv Biol 1997;11:251-255.

19.Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-2739.

20.Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596-1599.

21.Nei M, Kumar S. Molecular evolution and phylogenetics. Oxford University Press, New York. 2000; 420.

22.Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing evolutionary trees. Mol Biol Evol 1987;4:406-425.

23.Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10: 512-526.

24.Nei M. Molecular evolutionary genetics. Columbia University Press, New York, NY, USA. 1987; 350.

25.Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004;101:11030-11035.

26.Nassiri MR, Mahdavi M. Genetic and phylogenetic analysis of cytochrome b region in Jebeer of Iran. J Agr Bitech 2011;3:91-104.