Genetic population structure and differentiation of Western Iranian Oxynoemacheilus argyrogramma (Heckel, 1847) using SSR markers

Document Type: Original article

Authors

Aquatic Ecology, Gorgan University of Agricultural Sciences and Natural Resources, Iran

Abstract

This study was carried out to investigate the genetic diversity and population structure of 90 specimens of Oxynoemacheilus argyrogramma collected from Sepidbarg, Gamasiab and Ghaleji rivers, in the west of Iran. Analyses using three microsatellite loci indicated that the average number of alleles in the population was 12, which was well above the reported values for freshwater fishes. The expected (He) and observed (Ho) heterozygosity means were 0.865 and 0.576, respectively. Almost all loci showed deviation from the Hardy-Weinberg equilibrium (HWE). The results demonstrated that Oxynoemacheilus argyrogramma had desirable genetic diversity in the investigated regions.

Keywords


1.Askari GH, Shabani A. Genetic diversity evaluation of Paraschistura bampurensis (Nikolskii, 1900) in Shapour and Berim rivers (Iran) using microsatellite markers. Cell Biol and Gen 2013;3:29-34.

2.Esmaeili HR, Coad BW, Gholamifard A, Nazari N, Teimori A. Annotated checklist of the freshwater fishes of Iran. Zoosystematica Rossica 2010;19:361-386.

3.Kottelat M, Freyhof J. Handbook of European Freshwater Fishes. Cornol: Publications Kottelat. 2007.

4.Sediva A, Apostolou A, Kohout J, Bohlen J. Molecular phylogeographic analyses of the loach Oxynoemacheilus bureschi reveal postā€glacial range extensions across the Balkans. Fish Biol 2010;76:357-368.

5.Bataillon TM, David JL, Schoen DJ. Neutral genetic markers and conservation: Simulated germplasm collections. Genetics 1996;144:409-417.

6.Sun YF, Sun HY, Zhao Y, Qian GH, Zhao Y, Zhao Q, Liu NF. Isolation and characterization of microsatellite loci in the freshwater crab Sinopotamon yangtsekiense and cross-species amplification in related taxa (Decapoda: Brachyura). J Crustacean Biol 2009;29:273-274.

7.O'Connell M, Wright J M. Microsatellite DNA in fishes. Fish Biol 1997;7: 331-363.

8.Neff BD, Gross MR. Microsatellite evolution in vertebrates: inference from AC dinucleotide repeats. Evolution 2001;55:1717-1733.

9.Hillis DM, Mortiz C, Mable BK. Molecular systematic. Signature associated. 1996.

10.Taylor M, Blust R, Verheyen E. Characterization of microsatellite loci in the stone loach, Barbatula barbatula L. Mol Eco 2001;1:96-97

11.Peakall R, Smouse PE. GenAlex 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 2012;28:2537-2539.

12.Nei M. Estimation of average heterozygosity and genetic distance from small number of individuals. Genetics 1978;89:583-590.

13.Laloei F, Rezvani Gilkolaei S, Taghavi MJ. Genetic Diversity and Differentiation of Common Carp (Cyprinus carpio L.) in the southern part of Caspian Sea by using microsatellite markers. Asian Fish Sci 2013;26:115-127

14.Reed DH. When it comes to inbreeding: Slower is better. Mol Ecol 2009;18:4521-4522.

15.Gopalakrishnan A, Musammilu KK, Muneer PMA, Lal KK, Kapoor D, Ponniah AG, Mohindra V. Microsatellite DNA markers to assess population structure of red-tailed barb, Gonoproktopterus curmuca. Acta Zool 2004;50:686-690

16.Liu ZJ, Cordes FJ. DNA marker technologies and their applications in aquaculture genetics. Aquaculture 2004;238:1-37.

17.Dewoody JA, Avise JC. Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals. Fish Biol 2000;56:461-473.

18.Askari GH, Shabani A, Ghodsi Z, Nowferesti H. Genetic comparison of Oxynoemacheilus Kiabii (Golzarianpour, Abdoli & Frehof, 2011) from Gamasiab River in Kermanshah and Hamadan Province, using microsatellite markers. Modern Genet 2104;9:387-390.

19.Ruzzante DE, Taggart CT, Cook D. Spatial and temporal variation in the genetic composition of a larval cod (Gadus morhua) aggregation: cohort contribution and genetic stability. Can J Fish Aquat Sci 1996;53:2695-2705

20.Li D, Kang D, Yin N, Sun X. Microsatellite DNA marker analysis of genetic diversity in wild common carp (Cyprinus carpio L.) populations. Gen Genom 2007; 34:984-993.

21.Grassi F, Imazio S, Gomarasca S, Citterio S, Aina R, Sgorbati S, Sala F, Patrignani G, Labra M. Population structure and genetic variation within Valeriana wallrothii Kreyer in relation to different ecological locations. Plant Sci 2004;166:1437-1441.

22.Wright S. Evolution and the genetics of populations, vol. 4: Variability within and among natural populations. University of Chicago Press, Chicago.1987; p 590.