Association of CAT C-262T and SOD1 A251G single nucleotide polymorphisms susceptible to gastric cancer

Document Type: Original article

Authors

1 Shiraz University

2 Department of Biology, College of Sciences, Shiraz University

Abstract

Oxidative stress is known to be one of the major factors involved in the development and progression of cancer. Oxidative stress can occur due to an imbalance between concentrations of reactive oxygen species and antioxidant capacities. Catalase (CAT; OMIM 115500) and superoxide dismutase 1 (SOD1; OMIM 147450) play important roles in the primary defense against oxidative stress. In the present study, we investigated possible associations between polymorphisms of CAT C-262T (rs1001179) and SOD1 A251G (rs2070424) with susceptibility to gastric cancer. This case-control study included 160 gastric cancer patients and 241 age and gender frequency-matched healthy controls. Genotyping was done using PCR-RFLP based method. There were no significant differences in T allele frequencies in patients as compared to the controls in the CAT C-262T polymorphism (OR=0.80, 95% CI: 0.52- 1.23, P=0.304). Subjects with AG (OR=0.47, 95% CI: 0.24-0.91, P=0.026) or AG+GG (OR=0.45, 95% CI: 0.23-0.88, P=0.021) genotypes of the rs2070424 polymorphism were at lower risks of developing gastric cancer in comparison with the AA genotype. Our findings showed that there was no significant association between CAT C-262T polymorphism and gastric cancer susceptibility. However, we found that the G allele of the SOD1 A251G polymorphism has protective effects against the risk of gastric cancer.

Keywords


1.Koo SH, Jeong TE, Kang J, Kwon KC, Park JW, Noh SM. Prognostic implications for gastric carcinoma based on loss of heterozygosity genotypes correlation with clinicopathologic variables. Cancer Genet Cytogenet 2004;153:26-31.

2.Crew KD, Neugut AI. Epidemiology of gastric cancer. World J Gastroenterol 2006; 12:354- 362.

3.Ushijima T, Sasako M. Focus on gastric cancer. Cancer Cell 2004;5:121-125.

4.Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, 2002. CA Cancer J Clin 2005;55:74-108. 

5.Pourfarzi F, Whelan A, Kaldor J, Malekzadeh R. The role of diet and other environmental factors in the causation of gastric cancer in Iran. A population based     study. Int J Cancer 2009; 125:1953-1960.

6.Mehrabani D, Tabei SZ, Heydari ST, Shamsina SJ, Shokrpour N, Amini M, Masoumi SJ, Julaee H, Farahmand M, Manafi A. Cancer occurrence in Fars Province, southerm Iran. Iran Red Crescent Med J 2008;10:314-322.

7.Cover TL, Blaser MJ. Helicobacter pylori in health and disease. Gastroenterology2009;136:1863-1873.

8.Wroblewski LE, Peek RM Jr. Helicobacter pylori in gastric carcinogenesis mechanisms. Gastroenterol Clin North Am 2013;42:285-298.

9.González CA, Pera G, Agudo A, Palli D, Krogh V, Vineis P, Tumino R, Panico S, Berglund G, Simán H, Nyrén O, Agren A, Martinez C, Dorronsoro M, Barricarte A, Tormo MJ, Quiros JR, Allen N, Bingham S, Day N, Miller A, Nagel G, Boeing H, Overvad K, Tjonneland A, Bueno-De-Mesquita HB, Boshuizen HC, Peeters P, Numans M, Clavel-Chapelon F, Helen I, Agapitos E, Lund E, Fahey M, Saracci R, Kaaks R, Riboli E. Smoking and the risk of gastric cancer in the European Prospective Investigation Into Cancer and Nutrition (EPIC). Int J Cancer 2003;107: 629-634.

10.Shull S, Heintz NH, Periasamy M, Manohar M, Janssen YM, Marsh JP, Mossman BT. Differential regulation of antioxidant enzymes in response to oxidants. J Biol Chem 1991;266:24398-403.

11.McCord JM. Oxygen-derived free radicals. New Horiz 1993;1:70-76.

12.Fridovich I. Superoxide anion radical (O2-.), superoxide dismutases, and relatedmatters. J Biol Chem 1997;272:18515-18517.

13.Culotta VC. Superoxide dismutase, oxidative stress, and cell metabolism. Curr Top Cell Regul 2000;36:117-132.

14.Czeczot H, Scibior D, Skrzycki M, Podsiad M. Antioxidant barrier in patients with gastric cancer-preliminary study. Pol Merkur Lekarski 2005;19:521-525.

15.Hwang TS, Choi HK, Han HS. Differential expression of manganese superoxide dismutase, copper/zinc superoxide dismutase, and catalase in gastric adenocarcinoma and normal gastric mucosa. Eur J Surg Oncol 2007;33:474-479.

16.Monari M, Foschi J, Calabrese C, Liguori G, Di Febo G, Rizzello F, Gionchetti P, Trinchero A, Serrazanetti GP. Implications of antioxidant enzymes in human gastricneoplasms. Int J Mol Med 2009;24:693-700.

17.Ahn J, Nowell S, McCann SE, Yu J, Carter L, Lang NP, Kadlubar FF, Ratnasinghe LD, Ambrosone CB. Associations between catalase phenotype and genotype: Modification by epidemiologic factors. Cancer Epidemiol Biomarkers Prev 2006;15: 1217-1222.

18.Kang D, Lee KM, Park SK, Berndt SI, Peters U, Reding D, Chatterjee N, Welch R, Chanock S, Huang WY, Hayes RB. Functional variant of manganese superoxide dismutase (SOD2 V16A) polymorphism is associated with prostate cancer risk in the prostate, lung, colorectal, and ovarian cancer study. Cancer Epidemiol Biomarkers Prev 2007;16:1581-1586.

19.Yi Zhang, Lan Zhang, DongLin Sun, ZhiSheng Li, Lin Wang, Ping Liu. Genetic polymorphisms of superoxide dismutases, catalase, and glutathione peroxidase in age-related cataract. Mol Vis 2011;17:2325–2332.

20.Tiwari AK, Prasad P, B K T, Kumar KM, Ammini AC, Gupta A, Gupta R. Oxidative stress pathway genes and chronic renal insufficiency in Asian Indians with Type 2 diabetes. J Diabetes Complications 2009;23:102-111.

21.Rajaraman P, Hutchinson A, Rothman N, Black PM, Fine HA, Loeffler JS, Selker RG, Shapiro WR, Linet MS, Inskip PD. Oxidative response gene polymorphisms and risk of adult brain tumors. Neuro Oncol 2008;10:709-715.

22.Ahn J, Gammon MD, Santella RM, Gaudet MM, Britton JA, Teitelbaum SL, Terry MB, Nowell S, Davis W, Garza C, Neugut AI, Ambrosone CB. Associations between breast cancer risk and the catalase genotype, fruit and vegetable consumption, and supplement use. Am J Epidemiol 2005;162:943-952.

23.Amirshahi P, Sunderland E, Farhud DD, Tavakoli SH, Daneshmand P, Papiha SS. Serum proteins and erythrocyte enzymes of populations in Iran. Hum Hered 1989; 39:75-80.

24.Mohamadynejad P, Saadat M. Genetic polymorphisms of XRCC1 (at codons 194 and 399) in Shiraz population (Fars province, southern Iran). Mol Biol Rep 2008; 35:669-672.

25.Rafiee L, Saadat I, Saadat M. Glutathione S-transferase genetic polymorphisms (GSTM1, GSTT1 and GSTO2) in three Iranian populations. Mol Biol Rep 2010;37: 155-158.

26.Newton CR. Mutational analysis: Known mutations: McPherson MJ, Hames D, Taylor GR (Eds). PCR2. A Practical Approach. IRL Press Oxford 1995; 219-222.

27.Suzen HS, Gucyener E, Sakalli O, Uckun Z, Kose G, Ustel D, Duydu Y. CAT C- 262T and GPX1 Pro198Leu polymorphisms in a Turkish population. Mol Biol Rep 2010;37:87-92.

28.Zhang Y, Zhang L, Sun D, Li Z, Wang L, Liu P. Genetic polymorphisms of superoxide dismutases, catalase, and glutathione peroxidase in age-related cataract. Mol Vis 2011;17:2325-2332.

29.Yi JF, Li YM, Liu T, He WT, Li X, Zhou WC, Kang SL, Zeng XT, Zhang JQ. Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer. World J Gastroenterol 2010;16:4738-4746.

30.Ailawadhi S, Kardosh A, Yang D, Cozen W, Patel G, Alamgir MA, Chanan-Khan AA. Outcome disparities among ethnic subgroups of Waldenström's macroglobulinemia: a population-based study. Oncology 2014;86:253-262.