1Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
2Department of Biology, Faculty of Basic Science, Payamenoor University, Tehran, Iran.
3Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
4Department of Physiology, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Despite the preventative and therapeutic effects of regular exercise, exhaustive exercise may be harmful to health. The present study aimed to determine the protective effect of endurance training and cinnamon bark extract (CBE) supplementation on oxidative responses induced by an exhaustive exercise schedule in rats. The rats were randomly divided into the following five groups of 6; control sedentary (Con/Sed), control exercised (Con/Ex), trained exercised (Tr/Ex), supplemented exercised (Sup/Ex), and trained, supplemented and exercised (Tr/Sup/Ex). Animals in exercise groups ran on a rodent treadmill for an 8-week endurance training program. At the end of the experiment, blood samples were collected and (MDA) and total thiol (TT) levels were measured in plasma. Glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) activities were determined in soleus muscles. Results showed significant increases in SOD activity and malondealdehyde (MDA) levels in the soleus muscles and serum of exercised rats fed with the normal diet. The exhaustive exercise also induced a decrease in serum total thiol level and GPX activity. Elevated levels of total thiol and total antioxidant capacity (TAC) and reduced serum MDA levels were found in the Sup/Ex and Tr/Sup/Ex groups. CAT and GPX activities increased by CBE treatment in trained rats. Regular training increased CAT and GPX activities in the Tr/Sup/Ex group. CAT, GPX and SOD activities were not affected by the CBE treatment in untrained rats. Results suggest that additional use of regular training and CBE supplementation increase TAC and protect healthy male rats against oxidative damage induced by exhaustive exercise.
1.Helgerud J, Hoydal K, Wang E. Aerobic high- intensity intervals improve vo2 max more than moderate training. Med Sci Sport Exerc 2007; 39(4): 665- 671.
2.Gomez-Cabrera MC, Domenech E, Vina J. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic Biol Med 2007; 44: 126-131.
3.Aguilo A, Tauler P, Fuentesoina E, Tur JA, Cordova A, Pons A. Antioxidant response to oxidative stress induced by exhaustive exercise. Physiloghy and behavior 2005; 84: 1-7.
4.Hessel E, Haberland A, Muller M, Lerche D, Schimke I. Oxygen radical generation of neutrophils: a reason for oxidative stress during marathon running? Clin Chim Acta 2000; 298: 145– 56.
5.Alessio HM, Hagerman AE, Fulkerson BK, Ambrose R, Robyn E, Wiley R. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sport Exerc 2000; 32(9): 1576-1581.
6.Sacheck JM, Milbury PE, Cannon JG, Roubenoff R, Blumberg JB. Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. Free Radic Biol Med 2003; 34: 1575–1588.
7.Aguilo A, Tauler p, Fuentesoina E, Tur JA, Cordova A, Pons A. Antioxidant response to oxidative stress induced by exhaustive exercise. Physiol Behav 2005; 84: 1-7.
8.Scandalios J. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz J Med Bio Res 2005; 38: 995-1014.
9.Change CK, Huang HY, Tseng H, Hsuuw H, Tso T. Interaction of vitamin E and exercise training on oxidative stress and antioxidant enzyme activities in rat skeletal muscles. J Nutr Biochem 2006; 18: 39-45.
10.Dehghan G, Shafiee A, Ghahremani MH, Ardestani K, Abdollahi M. Antioxidant potential of various extract from ferula szovitsiana in relation to their phenolic content. Pharm Biol 2007; 45: 691-699.
11.Jamurtas AZ, Fatouros IG, Deliconstantinos G, Viliotou V, Fatinakis P, Magiria T, Tokmakidid S. Chronic endurance and resistance exercise effects on oxidative stress and antioxidant status of inactive older adults. Med Sci Sport Exerc 2003; 35(5).
12.Avellini L, Chiaradia E, Gaiti A. Effect of exercise training, selenium and vitamin E on some free radical scavengers in horses (Equus caballus). Comparative Biochemistry and Physiology Part B 1993; 147–154
13.Jayaprakasha GK, Negi PS, Jena BS, Jagan Mohan Roa L. Antioxidant and anti mutagenic activities of cinnamomum zeylancium fruit extracts. J Food Comp Anal 2006; 20: 330- 336.
14.Lan S, Jun-Jie Y, Denys Ch, Kequan Zh, Jeffrey M, Liangli Y. Total phenolic contents chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food Chem 2007; 100: 990–997
15.Gurdip S, Maurya S, Delampasona MP, Catalon C. A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food Chem Toxicol 2007; 45: 1650–1661
16.Nagendra N, Yang B, Dong X, Jiang G, Zhang H, Xie H, Jiang Y. Flavonoid contents and antioxidant activities from cinnamomum species. Innov food Sci Emerg Technol 2009; 10(4): 627-632.
17.Lee JS, Jeon SM, Park EM, Huh TL, Kwon OS, Lee MK, Choi MS. Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterol-fed rats. J Med Food 2003; 6(3): 183-191.
18.Ranjbar A, Ghaseminezhad S, Zamani H, Takalu H, Baiaty A, Rahimi F, Abdollahi M. Antioxidative stress potential of cinnamomum zeylancium in human: a comparative cross-sectional clinical study. Clin Pract 2006; 3(1): 113-117.
19.Jafari A, Hosseinpourfeizi MA, Hooshmand M, Ravasi AA, Montazeri M. Effect of aerobic exercise training on mtDNA deletion in soleus muscle of trained and untrained Wistar rats. Sport Sci Res Let 2003; 18: 97-115.
20.Moselhy S, Junbi H.Antioxidant properties of ethanolic and aqueous Cinnamon extracts against liver injury in rats. Int J Adv Pharm Sci 2010; 1: 151-155.
21.Pinho RA, Andrades ME, Olivera MR, Pirola AC, Zago MS, Silveira PC, Dal-Pizzol F, Moreira JC. Imbalance in SOD/CAT activities in skeletal muscles submitted to treadmill training exercise. Cell Biol Int 2006; 30: 848-853
22.Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as measure of antioxidant power. Anal Biochem 1996; 239: 70-76.
23.Beutler E, Duron O, Kelly B. Improved method for the determination of blood glutathione. J Lab Clin Med 1863; 61: 882–8.
24.Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-169.
25.Delmas-Beauvieux MC, Peuchant E, Dumon MF, Receveur MC, Le Bras M, Clerc M. Relationship between red blood cell antioxidant enzymatic system status and lipoperoxidation during the acute phase of malaria. Clin Biochem 1995; 28: 163-169.
26.Aebi H. Catalase in vitro. Methods Enzymol 1984; 105:121–126.
27.Huang CC, Tsai SS, Lin WT. Potential ergogenic effects of L-arginine against oxidative and inflammatory stress induced by acute exercise in aging rats, Exp Gerontol 2008; 43: 571- 577.
28.Miazaki h, Ookawara T, Kiazaki T, Haga S, Ji LL, Ohno H.Strenuous endurance training in humans reduces oxidative stress following exhausting exercise, Eur J Appl Physiol 2001; 84: 1-6.
29.Mashhadi NS, Ghiasvand R, Hariri M, Askari G, Feizi A, Darvishi L, Hajishafiee M, Barani A. Effect of ginger and cinnamon intake on oxidative stress and exercise performance and body composition in Iranian female athletes, Int J Prev Med 2013; 4: 531-5.
30.Metin G, Gumustas MK, Uslu E, Belce A and Kayserilioglu A. Effects of regular training on plasma thiols, malondialdehyde and carnitine concentrations in young soccer players. Chin J Physiol 2003; 46(1), (Abstract).
31.Simoes V, Panza P, Wazlawik E, Schutz GR, Comin L, Hecht KC, Luiz da Silva E. Consumption of green tea favorably affects oxidative stress markers in weight- trained men. Nutrition 2008; 24(5): 433- 442.
32.Dunlap KL, Reynolds AJ, Duffy KL. Total antioxidant power in sled dogs supplemented with blueberry comparison of blood parameters associated with exercise. Comp Biochem Phys A 2006; 143: 429-434.
33.Morrillas- Ruiz JM, Villegas Garcia JA, Lopez FJ, Vidal- Guevava ML, Zafrilla P. Effect of polyphenolic antioxidant on eexercise-induced oxidative stress. Clin Nutr 2006; 25(3): 444-453.
34.Ji LL. Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc 1993; 25: 225-231.
35.Gore M, Fiebig R, Hollander J, Ji LL. Acute exercise alters mRNA abundance of antioxidant enzyme and nuclear factor B activition in skeletal muscle, heart, and liver. Med Sci Sports Exerc 1997; 29: S229.
36.Gul M, Demircan B, Taysi S, Oztasan N, Gumustekin K, Siktar M, Polat F, Akar S, Akcay F, Dane S. Effect of endurance training and acute exhaustive exercise on antioxidant defense mechanisms in rat heart. Comp Biochem Physiol A Mol Integr Physiol 2006; 143(2): 239-245.
37.Tauler P, Sureda A, Cases N. Increased lymphocyte antioxidant defenses in response to exhaustive exercise do not prevent oxidative damage. J Nutr Biochem 2006; 17: 665-671.
Leeuwenburgh C, Hollander J, Leichtweis S, Griffiths M, Gore M, Ji LL. Adaptations of glutathione antioxidant system to endurance training are tissue and muscle fiber specific. Am J Physiol 1997; 272, R363– R369. Powers SK, Criswell D, Lawler J, Ji LL, Martin D, Herb RA, Dudley G. Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle. Am J Physiol 1994; 266, R375–R380.