Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran
Myricetin (Myc) is one of the most important flavonoids in diet due to its abundance in foods with the highest antioxidant activity. The antioxidant activity of Myc was studied in cell-free and cell-based systems to evaluate the ROS protection efficiency of Myc. The studies were based on the assessment of reducing power of Myc according to ferric ion reduction and intracellular ROS level measurement by assaying the cellular fluorescence intensity using dichlorodihydrofluorescein (DCF) probe as an indicator for ROS in cells. Moreover, the antitoxic capability of Myc was assessed using MTT method. Data indicated that intracellular ROS are highly toxic and applying low concentration of Myc not only inhibited cellular ROS production but also was accompanying with the protection of cells against the highly toxic and the lethal effects of peroxide compounds. Because of strong correlation between cellular ROS and their cell toxic properties, the higher antioxidant potency of Myc in cell medium resulted in effectively blocking intracellular ROS and protecting cell death. This property is achieved by the help of high polar solubility and cell membrane permeability of Myc.
1. Gee JM, Johnson IT. Polyphenolic compounds: interactions with the gut and implications for human health. Curr Med Chem 2001;8:1245-1255.
2. Kozłowska A, Szostak-Wegierek D. Flavonoids-food sources and health benefits. Rocz Panstw Zakl Hig 2014;65:79-85.
3. Kuhnau J. The flavonoids. A class of semi-essential food components: their role in human nutrition. World Rev Nutr Diet 1976;24:117-191.
4. Kandaswami C, Middleton E Jr. Free radical scavenging and antioxidant activity of plant flavonoids. Adv Exp Med Biol 1994;366:351-376.
5. Pollastri S, Tattini M. Flavonols: old compounds for old roles. Ann Bot 2011;108: 1225-1233.
6. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996;20:933-956.
7. Harnly JM, Doherty RF, Beecher GR, Holden JM, Haytowitz DB, Bhagwat S, Gebhardt S. Flavonoid content of U.S. fruits, vegetables, and nuts. J Agric Food Chem 2006;54:9966-9977.
8. Juhasz B, Varga B, Gesztelyi R, Kemeny-Beke A, Zsuga J, Tosaki A. Resveratrol: A multifunctional cytoprotective molecule. Curr Pharm Biotechnol 2010;11:810-818.
9. Phillips PA, Sangwan V, Borja-Cacho D, Dudeja V, Vickers SM, Saluja AK. Myricetin induces pancreatic cancer cell death via the induction of apoptosis and inhibition of the phosphatidylinositol 3-kinase (PI3K) signalling pathway. Cancer Lett 2011;308:181-188.
10. Chen W, Li Y, Li J, Han Q, Ye L, Li A. Myricetin affords protection against peroxynitrite-mediated DNA damage and hydroxyl radical formation. Food Chem Toxicol 2011;49:2439-2444.
11. Kang NJ, Jung SK, Lee KW, Lee HJ. Myricetin is a potent chemopreventive phytochemical in skin carcinogenesis. Ann N Y Acad Sci 2011;1229:124-132.
12. Li Y, Ding Y. Minireview: Therapeutic potential of myricetin in diabetes mellitus. Food Sci and Hum Wellness 2012;1:19-25.
13. Nöthlings U, Murphy SP, Wilkens LR, Henderson BE, Kolonel LN. Flavonols and pancreatic cancer risk: the multiethnic cohort study. Am J Epidemiol 2007;166: 924–931.
14. Kumamoto T, Fujii M, Hou DX. Myricetin directly targets JAK1 to inhibit cell transformation. Cancer Lett 2009;275:17-26.
15. Gordon MH, Roedig-Penman A. Antioxidant activity of quercetin and myricetin in liposomes. Chem Phys Lipids 1998;97:79-85.
16. Lee KW, Kang NJ, Rogozin EA, Kim HG, Cho YY, Bode AM, Lee HJ, Surh YJ, Bowden GT, Dong Z. Myricetin is a novel natural inhibitor of neoplastic cell transformation and MEK1. Carcinogenesis 2007;28:1918-1927.
17. Lin KH, Yang YY, Yang CM, Huang MY, Lo HF, Liu KC, Lin HS, Chao PY. Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes. BMC Res Notes 2013;6: 490.
18. Wang ZH, Ah Kang K, Zhang R, Piao MJ, Jo SH, Kim JS, Kang SS, Lee JS, Park DH, Hyun JW. Hyun JW. Myricetin suppresses oxidative stress-induced cell damage via both direct and indirect antioxidant action. Environ Toxicol Pharmacol 2010; 29:12-18.
19. Barzegar A, Pedersen JZ, Incerpi S, Moosavi-Movahedi AA, Saso L.The mechanism of antioxidant activity of IRFI005 as a synthetic hydrophilic analogue of vitamin E. Biochimie 2011;93:1880-1888.
20. Barzegar A. Proton-coupled electron-transfer mechanism for the radical scavenging activity of cardiovascular drug dipyridamole. PLoS One 2012;7:e39660.
21. Barzegar A. The role of electron-transfer and H-atom donation on the superb antioxidant activity and free radical reaction of curcumin. Food Chem 2012;135: 1369-1376.
22. Erdogan-Orhan I, Sever-Yılmaz B, Altun ML, Saltan G. Radical quenching activity, ferric-reducing antioxidant power, and ferrous ion-chelating capacity of 16 Ballota species and their total phenol and flavonoid contents. J Med Food 2010;13:1537-1543.
23. Brieger K, Schiavone S, Miller FJ Jr, Krause KH. Reactive oxygen species: from health to disease. Swiss Med Wkly. 2012;142: w13659.
24. Kirkinezosa IG, Moraes CT. Reactive oxygen species and mitochondrial diseases. Semin Cell Dev Biol 2001;12:449–457.