Establishing a new animal model for muscle regeneration studies

Document Type: Original article

Authors

1 Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran

2 Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Iran

3 Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

4 Department of Medical Laboratory Science, Medical Science Faculty Babol Islamic Azad University, Babol, Iran

5 Department of Radiology, Medical College of Wisconsin, Milwaukee, USA

6 Department of Developmental Biology, University of Science and Culture, Tehran, Iran

Abstract

Skeletal muscle injuries are one of the most common problems in the worldwide which impose a substantial financial burden to the health care system.  Accordingly, it widely accepted that muscle regeneration is a promising approach that can be used to treat muscle injury patients. However, the underlying mechanisms of muscle regeneration have yet to be elucidated. The muscle structure and muscle-related gene expression are highly conserved between human and zebrafish. Therefore, the zebrafish can be considered as an ideal animal model in muscle regeneration studies. In this study, Tol2 transposase was applied to produce Tg(mylpfa: cfp-nfsB) zebrafish model that express a fusion protein composed of cyan fluorescent protein (CFP) and nitrorudactase (NTR) under control of mylpfa promoter. The results showed that MTZ (Metronidazole) treatment of Tg(mylpfa:cfp-nfsB) zebrafish larvae can lead to muscle injury by selective ablation of muscle cells. And also, results confirmed the muscle regeneration ability of the transgenic larvae after withdrawal of Mtz for three days. Overall, The results of this study suggest that the Tg(mylpfa:cfp-nfsB) zebrafish model can be used in muscle regeneration study in order to elucidate the mechanisms of this process.

Keywords


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