Home Articles List Article Information
Molecular Biology Research Communications
Journal Archive
Volume Volume 8 (2019)
Volume Volume 7 (2018)
Volume Volume 6 (2017)
Volume Volume 5 (2016)
Volume Volume 4 (2015)
Volume Volume 3 (2014)
Volume Volume 2 (2013)
Volume Volume 1 (2012)
Ebrahimi-Askari, R., Behmanesh, M., Ghalandary, M., Soleimani, M. (2013). Nuclear factor-κB1 expression levels in human gastric adenocarcinoma. Molecular Biology Research Communications, 1(2), 57-64. doi: 10.22099/mbrc.2013.627
Razieh Ebrahimi-Askari; Mehrdad Behmanesh; Maryam Ghalandary; Masoud Soleimani. "Nuclear factor-κB1 expression levels in human gastric adenocarcinoma". Molecular Biology Research Communications, 1, 2, 2013, 57-64. doi: 10.22099/mbrc.2013.627
Ebrahimi-Askari, R., Behmanesh, M., Ghalandary, M., Soleimani, M. (2013). 'Nuclear factor-κB1 expression levels in human gastric adenocarcinoma', Molecular Biology Research Communications, 1(2), pp. 57-64. doi: 10.22099/mbrc.2013.627
Ebrahimi-Askari, R., Behmanesh, M., Ghalandary, M., Soleimani, M. Nuclear factor-κB1 expression levels in human gastric adenocarcinoma. Molecular Biology Research Communications, 2013; 1(2): 57-64. doi: 10.22099/mbrc.2013.627

Nuclear factor-κB1 expression levels in human gastric adenocarcinoma

Article 3, Volume 1, Issue 2, December 2012, Page 57-64  XML PDF (222 K)
Document Type: Original article
DOI: 10.22099/mbrc.2013.627
Authors
Razieh Ebrahimi-Askari1; Mehrdad Behmanesh 1; Maryam Ghalandary1; Masoud Soleimani2
1Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
22Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract
NF-κB pathway is a link between inflammation and cancer and is involved in cellular responses to different stimuli. Gastrointestinal lumen is exposed to many inflammatory agents such as foods, free radicals and bacterial or viral antigens. The aim of the present study was to evaluate the possible role of NF-κB1in gastric adenocarcinoma. To detect the relative level of NF-κB1transcript, total RNA was extracted from tissue specimens, a cDNA library was generated, and quantitative RT-PCR was performed for thirty human gastric adenocarcinoma tissue samples and thirty matched normal gastric tissue samples. NF-κB1 expression showed two-sidedness, which means that a group of 11 sample pairs showed up-regulation and a group of 16 sample pairs showed down-regulation. No histopathologic characteristics of samples could justify the observed two-sidedness. The NF-κB1 two-sidedness expression indicates the involvement of NF-κB1 and in a larger scale, NF-κB signaling pathway in gastric carcinogenesis. Our results show the complexity of regulatory mechanisms involved in developing and controlling the process of gastric cancer pathogenesis.
Keywords
Gastric cancer; NF-κB1; qRT-PCR; Gene expression
References
Malekzadeh R, Derakhshan MH, Malekzadeh Z. Gastric cancer in Iran: Epidemiology and risk factors. Arch Iran Med 2009;12:576-583.

  1. Smith MG, Hold GL, Tahara E, El-Omar EM. Cellular and molecular aspects of gastric cancer. World J Gastroenterol 2006;12:2979-2990.
  2. Lauren P. The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma: An attempt at a histo-clinical classification. Acta Pathol Microbiol Scand1965;64:31-49.
  3. Raskatov JA, Meier JL, Puckett JW, Yang F, Ramakrishnan P, Dervan PB, Modulation of NF-κB-dependent gene transcription using programmable DNA minor groove binders. Proc Natl Acad Sci USA 2012;109:1023-1028.
  4. Wang CY, Cusack JC, Liu R, Baldwin AS. Control of inducible chemoresistance: enhanced anti-tumor therapy through increased apoptosis by inhibition of NF-κB. Nat Med1999;5:412-417.
  5. Koch AE, Polverini PJ, Kunkel SL, Harlow LA, DiPi etro LA, Elner VM, Elner SG, Strieter RM. Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science1992;258:1798-1801.
  6. Kang MR, Kim MS, Kim SS, Ahn CH, Yoo NJ Lee SH. NF-kappa B signaling proteins p50/p105, p52/p100 RelA, and IKKepsilon are over-expressed in oesophageal squamous cell carcinomas. Pathology 2009;41:622-625.
  7. Karst AM, Gao K, Nelson CC, Li G. Nuclear factor kappa B subunit p50 promotes melanoma angiogenesis by upregulating interleukin-6 expression. Int J Cancer 2009;124: 494–501.
  8. Karin M, Cao Y, Greten FR, Li ZW. NF-κB in cancer: from innocent bystander to major culprit. Nat Rev Cancer 2002;2:301-310.
  9. Keates S, Hitti YS, Upton M, Kelly CP. Helicobacter pylori infection activates NF-κB in gastric epithelial cells. Gastroenterology 1977;113:1099-1109.
  10. Kim H, Lim JW, Kim KH. Helicobacter pylori-induced expression of interleukin-8 and cyclooxygenase-2 in AGS gastric epithelial cells: mediation by nuclear factor –kappa B. Scand J Gastroenterol 2001;36:706-716.
  11. Pasparakis M. Regulation of tissue homeostasis by NF-kappa B signaling: implications for inflammatory disease. Nat Rev Immunol 2009;9:778-788.
  12. Arora H, Qureshi R, Jin S, Park AK, Park WY. miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of  NF-κB1. Exp Mol Med 2011;43: 298-304.
  13. Setlur SR, Royce TE, Sboner A, Mosquera JM, Demichelis F, Hofer MD, Mertz KD,Gerstein M, Rubin MA. Integrative microarray analysis of pathways dysregulated in metastatic prostate cancer. Cancer Res 2007;67:10296-10303.
  14. Bazzoni F, Rossato M, Fabbri M, Gaudiosi D, Mirolo M, Mori L, Tamassia N, Mantovani A, Cassatella MA, Locati M. Induction and regulatory function of miR-9 in human monocytes and neutrophils exposed to proinflammatory signals. Proc Natl Acad Sci USA 2009;106:5282-5287.
  15. Wan HY, Guo LM, Liu T, Liu M, Li X, Tang H. Regulation of the transcription factor NF-kappaB1 by microRNA-9 in human gastric adenocarcinoma.  Mol Cancer 2010;9:16.
Statistics
Article View: 4,608
PDF Download: 4,787