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Ebrahimi-Askari, R., Behmanesh, M., Ghalandary, M., Soleimani, M. (2013). Nuclear factor-κB1 expression levels in human gastric adenocarcinoma. Molecular Biology Research Communications, 1(2), 57-64. doi: 10.22099/mbrc.2013.627
Razieh Ebrahimi-Askari; Mehrdad Behmanesh; Maryam Ghalandary; Masoud Soleimani. "Nuclear factor-κB1 expression levels in human gastric adenocarcinoma". Molecular Biology Research Communications, 1, 2, 2013, 57-64. doi: 10.22099/mbrc.2013.627
Ebrahimi-Askari, R., Behmanesh, M., Ghalandary, M., Soleimani, M. (2013). 'Nuclear factor-κB1 expression levels in human gastric adenocarcinoma', Molecular Biology Research Communications, 1(2), pp. 57-64. doi: 10.22099/mbrc.2013.627
Ebrahimi-Askari, R., Behmanesh, M., Ghalandary, M., Soleimani, M. Nuclear factor-κB1 expression levels in human gastric adenocarcinoma. Molecular Biology Research Communications, 2013; 1(2): 57-64. doi: 10.22099/mbrc.2013.627

Nuclear factor-κB1 expression levels in human gastric adenocarcinoma

Article 3, Volume 1, Issue 2, December 2012, Page 57-64  XML PDF (222 K)
Document Type: Original article
DOI: 10.22099/mbrc.2013.627
Authors
Razieh Ebrahimi-Askari1; Mehrdad Behmanesh 1; Maryam Ghalandary1; Masoud Soleimani2
1Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
22Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract
NF-κB pathway is a link between inflammation and cancer and is involved in cellular responses to different stimuli. Gastrointestinal lumen is exposed to many inflammatory agents such as foods, free radicals and bacterial or viral antigens. The aim of the present study was to evaluate the possible role of NF-κB1in gastric adenocarcinoma. To detect the relative level of NF-κB1transcript, total RNA was extracted from tissue specimens, a cDNA library was generated, and quantitative RT-PCR was performed for thirty human gastric adenocarcinoma tissue samples and thirty matched normal gastric tissue samples. NF-κB1 expression showed two-sidedness, which means that a group of 11 sample pairs showed up-regulation and a group of 16 sample pairs showed down-regulation. No histopathologic characteristics of samples could justify the observed two-sidedness. The NF-κB1 two-sidedness expression indicates the involvement of NF-κB1 and in a larger scale, NF-κB signaling pathway in gastric carcinogenesis. Our results show the complexity of regulatory mechanisms involved in developing and controlling the process of gastric cancer pathogenesis.
Keywords
Gastric cancer; NF-κB1; qRT-PCR; Gene expression
References
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