Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells

Document Type : Short communication

Authors

1 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

3 Department of Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran

Abstract

The Human endogenous retroviruses (HERVs) are ancient remnants of exogenous retroviral infections. Their abnormal activation is associated with several diseases, such as cancer and autoimmunity. Epigenetic and environmental factors are probably playing essential roles in the expression of these elements. This study aimed to examine the 96-hour effects of ELF-EMF on HERV-H, K, and W expression in human melanoma cells. SK-MEL-37 cells (the human skin malignant melanoma) were continuously exposed to ELF-EMF (50 Hz) at 1.5 and 3 mT intensity for 96 hours. Following mRNA extraction, the expression level of HERV-H, K, and W was assessed by qPCR. According to our results, exposure to ELF-EMF intensities for 96 hours could significantly downregulate HERV-H, K, and W env gene expression (P<0.001). Our obtained data suggest that low intensity and long-term exposure to ELF-EMF may pave using this type of radiation as a novel therapeutic approach by neutralizing the HERVs upregulated expression in melanoma cells.

Keywords

References

  1. van de Lagemaat LN, Landry J-R, Mager DL, Medstrand P. Transposable elements in mammals promote regulatory variation and diversification of genes with specialized functions. Trends Genet 2003;19:530-536.
  2. Karimi A, Madjd Z, Habibi L, Akrami SM. Evaluating the extent of LINE-1 mobility following exposure to heavy metals in HepG2 cells. Biol Trace Elem Res 2014;160:143-151.
  3. Young GR, Stoye JP, Kassiotis G. Are human endogenous retroviruses pathogenic? An approach to testing the hypothesis. Bioessays 2013;35:794-803.
  4. Grandi N, Tramontano E. Human Endogenous Retroviruses Are Ancient Acquired Elements Still Shaping Innate Immune Responses. Front Immunol 2018;9:2039.
  5. Meda F, Folci M, Baccarelli A, Selmi C. The epigenetics of autoimmunity. Cell Mol Immunol 2011;8:226-236.
  6. Krieg A, Gourley M, Perl A. Endogenous retroviruses: potential etiologic agents in autoimmunity. FASEB J 1992;6:2537-2544.
  7. Hohenadl C, Germaier H, Walchner M, Hagenhofer M, Herrmann M, Stürzl M, Kind P, Hehlmann R, Erfle V, Leib-Mösch C. Transcriptional activation of endogenous retroviral sequences in human epidermal keratinocytes by UVB irradiation. J Invest Dermatol 1999; 113:587-594.
  8. Hurst T, Pace M, Katzourakis A, Phillips R, Klenerman P, Frater J, Magiorkinis G. Human endogenous retrovirus (HERV) expression is not induced by treatment with the histone deacetylase (HDAC) inhibitors in cellular models of HIV-1 latency. Retrovirology 2016; 13:10.
  9. Karimi A, Sheervalilou R, Kahroba H. A new insight on activation of human endogenous retroviruses (HERVs) in malignant melanoma upon exposure to CuSO4. Biol Trace Elem Res 2019;191:70-74.
  10. Karimi A, Ghadiri Moghaddam F, Valipour M. Insights in the biology of extremely low-frequency magnetic fields exposure on human health. Mol Biol Rep 2020;47:5621-5633.
  11. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Non-ionizing radiation, Part 1: static and extremely low-frequency (ELF) electric and magnetic fields. IARC Monogr Eval Carcinog Risks Hum 2002;80:1-395.
  12. Grandi N, Tramontano E. HERV envelope proteins: Physiological role and pathogenic potential in cancer and autoimmunity. Front Microbiol 2018;9:462.
  13. Chiara C, Pitzianti MB, Matteucci C, D'Agati E, Miele MT, Rapaccini V, Grelli S, Curatolo P, Vallebona PS, Pasini A, Balestrieri E. The decrease in human endogenous retrovirus-H activity runs in parallel with improvement in ADHD symptoms in patients undergoing methylphenidate therapy. Int J Mol Sci 2018;19:3286.
  14. Dolei A, Ibba G, Piu C, Serra C. Expression of HERV genes as possible biomarker and target in neurodegenerative diseases. Int J Mol Sci 2019;20:3706
  15. Laska MJ, Nissen KK, Nexø BA (2013) Some cellular mechanisms influencing the transcription of human endogenous retrovirus, HERV-Fc1. PLoS One 2013;8:e53895.
  16. Liu Y, Liu W-b, Liu K-j, Ao L, Zhong JL, Cao J, Liu J-y. Effect of 50 Hz extremely low-frequency electromagnetic fields on the DNA methylation and DNA methyltransferases in mouse spermatocyte-derived cell line GC-2. Biomed Res Int 2015;237183.
  17. Mahmoudinasab H, Sanie-Jahromi F, Saadat M. Effects of extremely low-frequency electromagnetic field on expression levels of some antioxidant genes in human MCF-7 cells. Mol Biol Res Commun 2016;5:77-85.
  18. Del Re B, Marcantonio P, Gavoci E, Bersani F, Giorgi G. Assessing LINE-1 retrotransposition activity in neuroblastoma cells exposed to extremely low-frequency pulsed magnetic fields. Mutat Res 2012;749:76-81.
  19. Giorgi G, Pirazzini C, Bacalini MG, Giuliani C, Garagnani P, Capri M, Bersani F, Del Re B. Assessing the combined effect of extremely low-frequency magnetic field exposure and oxidative stress on LINE-1 promoter methylation in human neural cells. Radiat Environ Biophys 2017;56:193-200. 

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