The 40bp indel polymorphism of MDM2 increase the risk of cancer: An updated meta-analysis
Abdolkarim
Moazeni-Roodi
Department of Clinical Biochemistry, Iranshahr University of Medical Sciences, Iranshahr, Iran
author
Saeid
Ghavami
Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada
author
Mohammad
Hashemi
) Genetics of Non-communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
author
text
article
2019
eng
This meta-analysis aimed to provide an up-to-date comprehensive evaluation on the association between the MDM2 40bp indel polymorphism and cancer susceptibility. Eligible studies were retrieved by searching Web of Science, PubMed, Scopus, and Google scholar databases up to August 27, 2018. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to estimate the strength of association between the polymorphism and cancer risk. The findings of this meta-analysis revealed that the 40bp indel polymorphism significantly increased the risk of overall cancer risk in heterozygous (OR=1.06, 95%CI=1.01-1.11, P=0.016) and ID+DD (OR=1.07, 95%CI=1.01-1.14, P=0.027) genotypes. Stratified analysis by cancer type proposed that the study indel variant significantly associated with the risk of gastrointestinal cancer in heterozygous (OR=1.18, 95%CI=1.06-1.32, P=0.003) and ID+DD (OR=1.18, 95%CI=1.06-1.30, P=0.002) genotypes. The present findings showed a significant association between the MDM2 40bp indel polymorphism and overall cancer risk as well as gastrointestinal cancer susceptibility. Larger and well-designed researches are required to validate the findings association in detail.
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
1
8
https://mbrc.shirazu.ac.ir/article_5608_abcce1621f4219241924e2cbafd7c9d0.pdf
dx.doi.org/10.22099/mbrc.2019.31527.1364
Quantitative trait loci mapping of panicle traits in rice
Baoyan
Jia
Department of Agronomy, Shenyang Agricultural University, 110161, Shenyang China
author
Xinhua
Zhao
Department of Agronomy, Shenyang Agricultural University, 110161, Shenyang
author
Yang
Qin
Department of Agronomy, Shenyang Agricultural University, 110161, Shenyang China
author
Muhammad
Irfan
Department of Biotechnology, University of Sargodha, Sargodha 40100, Pakistan
author
Tae-heon
Kim
Department of Agronomy, Kyungpook National University, 702-701, Daegu, Republic of Korea
author
Bolun
Wang
Department of Agronomy, Shenyang Agricultural University, 110161, Shenyang China
author
Shu
Wang
Department of Agronomy, Shenyang Agricultural University, 110161, Shenyang China
author
Jae
Sohn
Department of Agronomy, Kyungpook National University, 702-701, Daegu, Republic of Korea
author
text
article
2019
eng
In this study 90 individuals of recombinant inbred lines (RILs) were developed by crossing subspecies of japonica rice cultivar, ‘Nagdong’ and an indica type cultivar, ‘Cheongcheong’. These individuals were used to identify the quantitative trait loci of panicle traits using SSR markers. A genetic linkage map was constructed using one hundred fifty four simple sequence repeat (SSR) primers covering distance of 1973.6 cM of the whole genome with mean distance of 13.9 cM among markers. QTLs were mapped using composite interval mapping method, nineteen QTLs were recognized for the panicle traits on chromosomes 4, 5, 6, 8, 10, 11, 12 with individual QTL explained 8.8% to 37.9% of phenotypic variation. Two pleiotropic effects loci were found on chromosomes 4 and 6. These QTLs affecting leaf traits, panicle traits and panicle branch traits would be beneficial to high-yield rice improvement.
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
9
15
https://mbrc.shirazu.ac.ir/article_5609_14589c5dcbf47fbe748b1fc0ec076444.pdf
dx.doi.org/10.22099/mbrc.2019.31550.1366
In silico analysis of suitable signal peptides for secretion of a recombinant alcohol dehydrogenase with a key role in atorvastatin enzymatic synthesis
Mortaza
Taheri
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
author
Seyyed Hossein
Khatami
Recombinant Proteins Laboratory, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
author
Zeinab
Jamali
Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
Amir
Savardashtaki
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
author
Younes
Ghasemi
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
author
Zohreh
Mostafavipour
Recombinant Proteins Laboratory, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
author
text
article
2019
eng
An elevated cholesterol level might lead to cardiovascular disease (CVD). Statins block the cholesterol synthesis pathway in the liver. Atorvastatin is the most widespread statin worldwide and, its chemical synthesis requires toxic catalysts, resulting in environmental pollution. Hence, enzymatic synthesis of atorvastatin is desirable. This process could be done by Lactobacillus kefir alcohol dehydrogenase (LKADH). Therefore, recombinant enzyme secretion by Escherichia coli using signal peptides (SPs) might result in easy production and purification. To achieve this objective, we used some online bioinformatics web servers to evaluate the suitable SPs for translocation of LKADH into extracellular spaces. “Signal Peptide Website” and “UniProt” were utilized to retrieve the SPs and LKADH sequences. “SignalP 4.1” was used to determine SPs and their cleavage site location and the results were rechecked by “Philius”. Physicochemical features of SPs were evaluated by “ProtParam”, then solubility of their fusion with LKADH was assessed by “Protein-sol”. Finally, secretion pathway and sub-cellular localization of the selected stable and soluble LKADH fusions were predicted by “PRED-TAT” and “ProtCompB”. Amongst the 41 evaluated SPs, only LPTA_ECOLI, SUBF_BACSU, CHIS_BACSU, SACB_BACAM, CDGT_BACST and AMY_BACLI could translocate LKADH out of cytoplasm. The six selected SPs in the result section were suitable to design a soluble secretory LKADH that accelerate its scale-up production and might be useful in future experimental researches.
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
17
26
https://mbrc.shirazu.ac.ir/article_5610_7a1f0ea000540a54fe826e984113e59c.pdf
dx.doi.org/10.22099/mbrc.2019.31801.1372
Genetic polymorphism in ERCC5 and breast cancer risk
Saima Shakil
Malik
Microbiology and Biotechnology Research Lab, Fatima Jinnah Women University, The Mall Rawalpindi
author
Sumaira
Mubarik
Department of Epidemiology and Biostatistics, School of Health Sciences, Wuhan University, Wuhan, China
author
Mehreen
Baig
Surgical Unit, Fauji Foundation Hospital, Rawalpindi
author
Nosheen
Masood
Microbiology and Biotechnology Research Lab, Fatima Jinnah Women University, The Mall Rawalpindi
author
Nayyer
Chaudhry
Armed Forces Institute of Pathology, Rawalpindi
author
text
article
2019
eng
ERCC5 plays crucial role in excision repair DNA damage induced by UV in NER pathway. Single neuleotide polymorphism in ERCC5 were responsible for different cancers.Therefore, current study evaluated the relationship between ERCC5 (rs1047768 T>C) polymorphism and the risk of breast cancer in Pakistani population. The rs1047768 polymorphism was screened among 175 females including one hundred breast cancer cases and age matched seventy-five healthy controls. Genotyping was performed with Tetra amplification-refractory mutation system (ARMS) PCR and products were observed through electrophoresis. Multivariate logistic regression was used to calculate odds ratio (OR) and 95% confidence interval (95% CI) investigating relationship between genotypes, clinical parameters and risk of breast cancer. Statistical analysis exhibited significant relationship between the TC genotype (OR=7.2, 95% CI=1.5-34.3) and increased breast cancer risk. Moreover, family history (OR=6.25; 95% CI= 2.61-15.00) and late menopause (OR=2.41; 95% CI=1.20-4.83) were found to be breast cancer associated risk factors. In conclusion, ERCC5 (rs1047768 T>C) polymorphism may contribute towards increased risk of breast cancer in Pakistani population.
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
27
31
https://mbrc.shirazu.ac.ir/article_5611_5c843fcb0aac0c14428a07ccc7956196.pdf
dx.doi.org/10.22099/mbrc.2019.32294.1383
A rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
Teena
Sajan
Credora Life Sciences, Horamavu, Bangalore, Karnataka-560043, India
author
Smitha
Murthy
Credora Life Sciences, Horamavu, Bangalore, Karnataka-560043, India
author
Rijesh
K
Credora Life Sciences, Horamavu, Bangalore, Karnataka-560043, India
author
Joyeeta
Mitra
Credora Life Sciences, Horamavu, Bangalore, Karnataka-560043, India
author
text
article
2019
eng
The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression based analysis using Real time PCR. The study showed that the mRNA levels are much lesser in patients suffering from dysplastic and metastatic tumors as compared to healthy individuals (P≤0.05). This study can be a breakthrough in medical and dentistry studies. One of the most common malignant carcinomas, OSCC is a type of cancer of the mouth. Though surgical methods have been quite effective in delaying the metastasis, the detection methods using histology parameters are not very efficient and the disease is diagnosed generally in the last stages of the cancer. Tetranectin is a protein biomarker which has been used for detection of several cancers including oral cancer where the protein quantity is calculated.
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
33
40
https://mbrc.shirazu.ac.ir/article_5612_9a28ffbab4abc2c90036ca9314a79e35.pdf
dx.doi.org/10.22099/mbrc.2019.31544.1365
Production and partial purification of thermostable bacteriocins from Bacillus pumilus ZED17 and DFAR8 strains with antifungal activity
Shahrzad
Dehghanifar
Department of Biotechnology, Faculty of Advanced Sciences & Technologies, University of Isfahan, Isfahan 81746-73441, Iran
author
Mehrnaz
Keyhanfar
Department of Biotechnology, Faculty of Advanced Sciences & Technologies, University of Isfahan, Isfahan 81746-73441, Iran
author
Giti
Emtiazi
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, 8146-73441, Iran
author
text
article
2019
eng
The bacteria which are members of the genus Bacillus are known to produce a wide variety of antimicrobial substances and bacteriocins. The main objective of this study was to investigate the effect of these bacteriocins on eukaryotic cells such as fungi, yeast and plant seeds. Several strains were screened for antifungal activities and identified by the means of polymerase chain reaction (PCR) of the 16s rRNA gene and sequencing. Our experiments showed that the Bacillus pumilus ZED17 and DFAR8 strains, had antifungal activities against Rhizoctonia solani and selected for further investigations. Extracellular peptides produced by these strains were purified by ammonium sulfate precipitation and dialysis. Addition of these peptides to Potato Dextrose Agar (PDA) medium inoculated with R. solani indicated significant inhibition of the fungal growth. The antifungal peptides were thermo-stable and remained active after boiling at 100˚C for 15 min. The molecular weight of the peptide with antifungal activity was estimated by electrophoresis on the Sodium Dodecyl Sulfate Poly Acrylamide Gel (SDS-PAGE) as about 5 KDa. Structural nature of this peptide was determined after gel extraction by Fourier-transform infrared spectroscopy (FTIR). Moreover, this peptide showed inhibiting effect on seeds germination of some herbs. This peptide could be applied to control herbal fungal disease induced by R. solani which is a broad host range plant pathogen fungus and its inhibition is very important. The peptide also prevents seed germination. Hence, it can be appropriate for inhibiting weeds growth. No significant effect against Saccharomyces cerevisiae and Candida albicans was observed.
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
41
49
https://mbrc.shirazu.ac.ir/article_5613_3104a67fd008b71bbc795567cdf32436.pdf
dx.doi.org/10.22099/mbrc.2019.31563.1367
Retraction Note: Association between T-786C polymorphism of endothelial nitric oxide synthase gene and level of the vessel dilation factor in patients with coronary artery disease
Journal
Admin
Molecular Biology Research Communications
author
text
article
2019
eng
Molecular Biology Research Communications
Shiraz University Press
2322-181X
8
v.
1
no.
2019
51
51
https://mbrc.shirazu.ac.ir/article_5614_ae7d40df013b1899e228b3854799a6f0.pdf
dx.doi.org/10.22099/mbrc.2019.32888.1389