ORIGINAL_ARTICLE
The binding assessment with human serum albumin of novel six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands
The interactions between platinum complexes and human serum albumin (HSA) play crucial roles in the distribution, metabolism, and activity of platinum-based anticancer drugs. Octahedral platinum (IV) complexes represent a significant class of anticancer agents that display molecular pharmacological properties different from cisplatin. In this study, the interaction between two Pt(IV) complexes with the general formula [Pt(X)2Me2 (tbu2bpy)], where tbu2bpy = 4,4′-ditert-butyl-2,2′-bipyridine, with two leaving groups of X = Cl (Com1) or Br (Com2), and HSA were investigated, using Ultraviolet-Visible (UV-Vis) spectroscopy, fluorescence spectroscopy, circular dichroism (CD) and molecular docking simulation. The spectroscopic and thermodynamic data revealed that the HSA/Pt(IV) complexes interactions were spontaneous process and Com2 demonstrated stronger interaction and binding constant in comparison with Com1. Also, the results suggest approximately similar structural alteration of HSA in the presence of these Pt complexes. Molecular docking revealed that both Pt(IV) complexes bind with HSA in subdomain IB, literally the same as each other. This study suggests that variation in the leaving group, displaying differing departure rate, has no significant contribution in denaturing prosperities of the Pt(IV) complexes against HSA.
https://mbrc.shirazu.ac.ir/article_3165_ecc2d55b361bb567ec45b604cd1fd6ba.pdf
2015-12-01
167
179
10.22099/mbrc.2015.3165
Human serum albumin
Platinum (IV) complexes
Spectroscopic studies
Molecular docking simulation
Reza
Yousefi
ryousefi@shirazu.ac.ir
1
Protein Chemistry Laboratory (PCL), Department of Biology, Shiraz University, Shiraz, Iran. Institute of Biotechnology, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
Asghar
Taheri-Kafrani
a.taheri@ast.ui.ac.ir
2
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran
AUTHOR
Sayed Masoud
Nabavizadeh
nabavi@chem.susc.ac.ir
3
Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran
AUTHOR
Zahra
Pouryasin
m.shahsavani@sci.uok.ac.ir
4
Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
AUTHOR
Mohammad Bagher
Shahsavani
mbagher_shahsavani@hotmail.com
5
Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
AUTHOR
Kazem
Khoshaman
kazem96@yahoo.com
6
Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
AUTHOR
Mehdi
Rashidi
rashidi@susc.ac.ir
7
Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran
AUTHOR
Wang X, Guo Z. Towards the rational design of platinum (II) and gold (III) complexes as antitumour agents. Dalton Trans 2008; (12):1521-1532.
1
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3
Kostova I. Platinum complexes as anticancer agents. Recent Pat Anticancer Drug Discov 2006; 1(1):1-22.
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Hall MD, Amjadi S, Zhang M, Beale PJ, Hambley TW. The mechanism of action of platinum (IV) complexes in ovarian cancer cell lines. J Inorg Biochem 2004; 98(10):1614-1624.
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7
Pendyala L, Kidani Y, Perez R, Wilkes J, Bernacki RJ, Creaven PJ. Cytotoxicity, cellular accumulation and DNA binding of oxaliplatin isomers. Cancer Lett 1995; 97(2):177-184.
8
Carter DC, Ho JX. Structure of serum albumin. Adv Protein Chem. 1994; 45:153-203.
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Fasano M, Curry S, Terreno E, Galliano M, Fanali G, Narciso P, Notari S, Ascenzi P. The extraordinary ligand binding properties of human serum albumin. IUBMB Life 2005; 57(12):787-796.
11
Ahmed-Ouameur A, Diamantoglou S, Sedaghat-Herati MR, Nafisi S, Carpentier R, Tajmir-Riahi HA. The effects of drug complexation on the stability and conformation of human serum albumin: protein unfolding. Cell Biochem Biophys 2006; 45(2):203-213.
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Bolli A, Marino M, Rimbach G, Fanali G, Fasano M, Ascenzi P. Flavonoid binding to human serum albumin. Biochem Biophys Res Commun 2010; 398(3):444-449.
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Brown JR, Shockley P. Serum albumin: structure and characterization of its ligand binding sites. New York: Wiley; 1982: 25-68.
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Hill GS, Vittal JJ, Puddephatt RJ. Methyl (hydrido) platinum (IV) complexes:  X-ray structure of the first (μ-Hydrido) diplatinum (IV) complex. Organometallics 1997; 16(6):1209-1217.
15
Kelly ME, Gómez-Ruiz S, Kluge R, Merzweiler K, Steinborn D, Wagner C, Schmidt H. Studies of mononuclear and dinuclear complexes of dibromodimethylplatinum(IV): preparation, characterization and crystal structures. Inorganica Chimica Acta 2009; 362(4):1323-1332.
16
Hu Y-J, Liu Y, Wang J-B, Xiao X-H, Qu S-S. Study of the interaction between monoammonium glycyrrhizinate and bovine serum albumin. J Pharm Biomed Anal 2004;36(4):915-919.
17
Divsalar A, Saboury AA, Yousefi R, Moosavi-Movahedi AA, Mansoori-Torshizi H. Spectroscopic and cytotoxic studies of the novel designed palladium (II) complexes: β-Lactoglobulin and K562 as the targets. Int J Biol Macromol 2007; 40(4):381-386.
18
Thomsen R, Christensen MH. MolDock:  A new technique for high-accuracy molecular docking. J Med Chem 2006; 49(11):3315-3321.
19
Keshavarz FA, M. M. Yousefi, R. Molecular dynamics simulation and docking studies on the binding properties of several anticancer drugs to human serum albumin. Mol Biol Res Commun 2012; 1(2):65-73.
20
Yotsuyanagi T, Ohta N, Futo T, Ito S, Chen DN, Ikeda K. Multiple and irreversible binding of cis-diamminedichloroplatinum(II) to human serum albumin and its effect on warfarin binding. Chem Pharm Bull (Tokyo) 1991; 39(11):3003-3006.
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Ohta N, Yotsuyanagi T, Chen D, Ono R, Ito S, Ikeda K. Disulfide bond cleavage of human serum albumin and alterations of its secondary structure by cis-diamminedichloroplatinum(II). Int J Pharm 1992; 85(1–3):39-44.
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Zhmareva EN, Zegzhda GD, Kas'ian GB, Livenskaia OA. Interaction of proteins with platinum and palladium compounds with differing biological activities. Ukr Biokhim Zh (1978) 1996; 68(3):74-79.
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Trynda-Lemiesz L, Kozlowski H, Katsaros N. Interaction of cis- and trans-RuCl (2)(DMSO)(4) with human serum albumin. Met Based Drugs 2000;7(6):293-299.
24
Bischin C, Lupan A, Taciuc V, Silaghi-Dumitrescu R. Interactions between proteins and platinum-containing anti-cancer drugs. Mini Rev Med Chem 2011;11(3):214-224.
25
Pouryasin Z, Yousefi R, Nabavizadeh SM, Rashidi M, Hamidizadeh P, Alavianmehr MM, Moosavi-Movahedi AA. Anticancer and DNA binding activities of platinum (IV) complexes; importance of leaving group departure rate. Appl Biochem Biotechnol 2014;172(5):2604-2617.
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28
Qian Y, Zhou X, Chen J, Zhang Y. Binding of bezafibrate to human serum albumin: insight into the non-covalent interaction of an emerging contaminant with biomacromolecules. Molecules 2012;17(6):6821-6831.
29
ORIGINAL_ARTICLE
Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon.
https://mbrc.shirazu.ac.ir/article_3166_004ad7f4d0e422dda0d9ed4b9d3af5a1.pdf
2015-12-01
181
188
10.22099/mbrc.2015.3166
Siberian sturgeon
Growth hormone
mRNA expression
RT-PCR
Zeinab
Abdolahnejad
z.abdolahnezhad@gmail.com
1
Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran.
LEAD_AUTHOR
Mohammad
Pourkazemi
pourkazemi@ifro.ir
2
Iran Fishery Research Organization (IFRO), Tehran, Iran.
AUTHOR
Majid Reza
Khoshkholgh
majidrezagu@yahoo.com
3
Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran.
AUTHOR
Mahtab
Yarmohammadi
mahtabyarmohammadi@gmail.com
4
International Sturgeon Research Institute, Rasht-Iran.
AUTHOR
Pourkazemi M. Caspian Sea sturgeon conservation and fisheries: Past, and Future. J Appl Ichthyol 2006; 22: 12-16.
1
Williot P, Arlati G, Chebanov M, Gulyas T, Kasimov R, Kirschbaum F, Patriche N, Pavlovskaya LP, Poliakova L, Pourkazemi M, Kim Y, Zhuang P, Zholdasova I. Status and management of Eurasian sturgeon: an overview. Internat. Rev. Hydrobiol 2002; 87:483-506.
2
Perez-Sanchez J, Smel J, Le Bail PY. Lacation and characterizeation of growth hormone binding sites in the central nervous system of a teleost fish (Oncorhynchus myksis). Growth Regul 1991; 1: 145-152.
3
Sakamoto T, Hirano T. Growth Hormone receptors in the liver and osmoregulatory organs of rainbow trout: characterization and dynamics during adaptation to seawater. J Endocrinol 1991; 130: 425-433.
4
Bjornsson BTH.The biology of salmon growth hormone: form daylight to dominance. Fish Physiol Biochem 1997; 17: 9-24.
5
Bjornsson BTH, Johansson V, Benedet S, Einarsdottir IE, Hildahl J, Agustsson T, Jonsson E. Growth hormone endocrinology of salmonids: regulatory mechanisms and mode of action. Fish Physiol Biochem 2004; 17:9-242
6
Perez-Sanchez J. The involvement of growth hormone in growth regulation, energy homeostasis and immunefunction in the Gilthead Sea bream (Sparus aurata). Fish Physiol Biochem 2000; 22:135-144.
7
Peter RE, Marchant TA. The endocrinology of growth in carp and related species. Aquaculture 1995; 129: 299-321.
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Funkenstein B, Cohen I. Ontogeny of growth hormone protein and mRNA in the gilthead sea bream Sparus aurata. Growth Regul 1996; 6:16-21.
9
Yang BY, Greene M, Chen TT. Early embryonic expression of the growth hormone family protein genes in the developing rainbow trout, Oncorhynchus myksis. Mol Reprod Dev1999; 53: 127-134.
10
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Li WS, Chen D,Wohg AO, Lin HR. Molecular cloning, tissue distribution, and ontogeny of mRNA expression of growth hormone in orange-spotted grouper (Epinephelus coioides). Gen Comp Endocrinol 2005; 144: 78-89.
12
Revol A, Rodriguez MLG, Montenegro VH, Aguilera C, Saldana HB, Mendoza R. Cloning of the growth hormone cDNA of alligator gar Atractosteus spatula and its expression through larval development. Comp Biochem Physiol 2005; 140: 423-429.
13
Ozaki Y, Fukada H, Tanaka H, Kagawa H, Ohta H, Adachi H, Akihiko H, Yamauchi K. Expression of Growth hormone family and growth hormone receptor during early development in the Japanese eel (Anguilla japonica). Comp Biochem Physiol 2006; 145: 27-34.
14
Cao H, Zhou RJ, Wei QW, Li CG, Gui JF. Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages. J Appl Ichthyol 2011; 27: 501-504.
15
Kolangi Miandare H, Farahmand H, Akbarzadeh A, Ramezanpour S, Kaiya H, Miyazato M, Rytkönen KT, Nikinmaa M. Developmental transcription of genes putatively associated with growth in two sturgeon species of different growth rate. Gen Comp Endocrinol 2013; 182:41–47.
16
Pourdehghani M, kazemi R, Yazdani MA, Pourkazemi M, Vahabzade H, Nazari RM, Behmanesh SH. Investigation of dosages suitable hormone LHRH-A2 and optimal conditions on the artificial propagation of sturgeon breeding stocks cultured of native and non-native of Iran. Aquaculture: In proceedings of the 1st Iranian Aquaculture symposium. Iran, Rasht, Anzali 2011.pp. 430- 434.
17
Chomczynski P, Sacchi N. Single-Step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction. Anal Biochem 1987; 162: 156-159.
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Akbarzade A, Farhmand H, Mahjoubi F, Nematollahi MA, Leskinen P, Rytkonen K, Nikinmaa M. The transcription of 1 - gulono -gamma - lactonoxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.Comp Biochem Physiol B Biochem Mol Biol 2011; 158: 282-288.
19
Radonic A, Thulke S, Mackay I M, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real–time PCR. Biochem Biophys Res Commun 2004; 313:856-873.
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Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-(ΔΔct) Method. Methods 2001; 25: 402-408.
21
Golos TG, Durining M, Fisher JM, Fowler PD. Cloning of four growth hormone / chorionic somatomammo tropin-related complementary deoxyri- bonucleic acids differentially expressed during pregnancy in the rhesus monkey placenta. Endocrinology 1993; 133: 1744-1752.
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Lacroix MC, evinoy ED, Servely JL, Puissan C, Kann G. Expression of the growth hormone gene in ovine placenata: detection and cellular localization of the growth hormone gene in ovine placenta: detection and cellular localization of the protein. Endocrinology 1996; 137: 4886-4892.
23
Frankenne F, Closset J, Gomez F, Scippo M L, Smal J, Nsnnen GH. The physiology of growth hormones (GHS) in pregnant women and partial characterization of the placental GH variant. J Clin Endocrinol Metab 1988; 66: 1171-1180.
24
Ostaszewska T, Dabrowski T. Early development of Acipenseriformes (Chondrostei, Actinopterygii), in: Y. W. Kunz, C. A. Luer, B.G. Kapoor (Ed.).Development of Non-teleost Fishes. Science publishers 2009; USA, PP: 170-229.
25
Gisbert E, Sarasquete M C, Wiolliot P, Castello-Orvay F. Histochemistery of the development of the digestive system of Siberian sturgeon during early ontogeny. J fish Biol 1999; 555: 96-616.
26
Gisbert E. Early development and allometric growth patterns in Siberian sturgeon and their ecological significance. J Fish Biol 1999; 54: 825-826.
27
Rodrlguez A, Gisbert E. Eye development and the role of vision during Siberian sturgeon early ontogeny. J Appl Ichthyol 2002; 18: 280-285.
28
Gisbert E, Williot P, Castello-Orvay F. Influence of egg size on growth and survival of early stages of Siberian sturgeon (Acipenser baerii) under small scale hatchery conditions. Aquaculture 2000; 183: 83-94.
29
Gisbert E, Rodrlguez A, CastelloOrvay F, Williot P. A histological study of the development of digestive tract of Siberian sturgeon (Acipenser baerii) during early ontogeny. Aquaculture 1998; 167: 195-209.
30
Szcepkowski M, Kolman R, Szczepkowska B. Postembryonic Development, Survival and growth rate of Siberian sturgeon (Acipenser baeri Brandt) Larvae. Arch Ryb Pol 2000; 8: 193-204.
31
ORIGINAL_ARTICLE
Molecular systematics and distribution review of the endemic cyprinid species, Persian chub, Acanthobrama persidis (Coad, 1981) in Southern Iran (Teleostei: Cyprinidae)
The Iranian Persian chub is an endemic species of the family Cyprinidae known only from few localities in drainages of Southern Iran. It was originally described in the genus Pseudophoxinus as (Pseudophoxinus persidis) and then Petroleuciscus (as Petroleuciscus persidis). In this study, we examined phylogenetic relationships of the Iranian Persian chubwith other relatives in the family Cyprinidae based on the mitochondrial cytochrome b gene to estimate the phylogenetic (and taxonomic) position of the species. Our molecular phylogenies show that new fish sequences from the drainages in southern Iran are clustered with sequences of the genus Acanthobrama from GenBank while the sequences from two other genera (Pseudophoxinus and Petroleuciscus) are in distinct clade. Therefore, we conclude that the populations of Persian Chub in drainages of southern Iran (i.e., Kol, Kor, Maharlu and Persis) belong to the genus Acanthobrama andspecies Acanthobrama persidis. The predicted geographic distributions for the species showed a large area of suitable climate for A. persidis across south and west of Iran especially in the Kor River basin. Some other parts in the Persis and Tigris are also might have been suitable habitats for this cyprinid species showing possible dispersal route of Acanthobrama from Tigris to the Persis, Kor and Kol basins.
https://mbrc.shirazu.ac.ir/article_3173_0a41e187c5794418cbd45cd917a5fb43.pdf
2015-12-01
189
206
10.22099/mbrc.2015.3173
mt-DNA
Persian Chub
Pseudophoxinus
Petroleuciscus
Iranian drainages
Azad
Teimori
azad.teimori@googlemail.com
1
Kerman University
AUTHOR
Hamid Reza
Esmaeili
hresmaeili22@gmail.com
2
Department of Biology,
College of Sciences,
Shiraz University
LEAD_AUTHOR
Golnaz
Sayyadzadeh
g.sayyadzadeh92@gmail.com
3
Shiraz University
AUTHOR
Neda
Zarei
ne63da@gmail.com
4
Shiraz University
AUTHOR
Ali
Gholamhosseini
gholamhosseini@gmail.com
5
Shiraz University
AUTHOR
Esmaeili HR, Coad BW, Gholamifard A, Nazari N, Teimori A. Annotated checklist of the freshwater fishes of Iran. Zoosyst Rossica 2010;19:361-386.
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62
ORIGINAL_ARTICLE
Description of a myxozoan parasite Myxobolus sp.n PKB2014 from an edible fish, with emphasis on its molecular characterization
In India, more than 104 species of Myxobolus have so far been reported infecting freshwater as well as marine fishes. The study focuses on the description of a new myxosporean species, Myxobolus sp.n PKB 2014 from the gill lamellae of an Indian major carp Labeo rohita. The species have been described on the basis of morphological characterization of the spores, tissue architecture and 18S rDNA sequence data. The plasmodia of Myxobolus sp.n PKB 2014 were round in shape measuring, 50 to 70 μm in diameter and spores were ellipsoidal in frontal view measures 14.7 ± 0.51 μm. The 18S rRNA nucleotide sequence with 806 bp of Myxobolus sp.n PKB 2014 (Accession number KJ652226) clustered phylogenitically with other Myxobolus spp. infecting cyprinid gills with 90-99% similarity. According to the phylogenetic study we concluded that M. wulli was the closest relative having 99% similarity with the species under description but the sequence was distinct in each species which additionally exhibited different morphological features. The infection rate was low to moderate. After through comparison it can be concluded that the species being described here is new to science which is designated as Myxobolus sp.n.PKB 2014.
https://mbrc.shirazu.ac.ir/article_3179_028448edd3b98239227dfaa8daaa6332.pdf
2015-12-01
207
216
10.22099/mbrc.2015.3179
Myxobolus sp.n PKB2014
West Bengal
Labeo rohita
18S rRNA
Phylogenetic relationship
Somerita
Panda
someritapanda@gmail.com
1
University of Kalyani
AUTHOR
Subarna
Ghosh
subarna_ghosh86@hotmail.com
2
University of Kalyani
AUTHOR
Probir Kumar
Bandyopadhyay
prabir0432@hotmail.com
3
UNIVERSITY OF KALYANI
LEAD_AUTHOR
Boreham RE, Hendrick S, O’Donoghue PJ, Stenzel DJ.Incidental finding of Myxobolus spores (Protozoa: Myxozoa) in stool samples from patients with gastrointestinal symptoms. J Clin Microbiol 1998;36:3728-3730.
1
Eiras JC, Molnár K, Lu YS. Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 2005;61:1-46.
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Lom J, Dyková I. Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 2006;53:1-36.
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Bütschli O. Myxosporidea. In: Bornn’s Klass Ordn., des Tierreiches, Protozoa. 1882; 1:590- 603
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Kalavati C, Nandi NC. Handbook of Myxosporidean parasites of Indian fishes. India, 2007;Kolkata: ZSI.
5
Bandyopadhyay PK, Hemananda T, Mitra AK, Mohilal N. Myxobolus dhanachandi sp. n. (Myxozoa, Myxosporea, Bivalvulida) from an Indian freshwater fish Channa orientalis (Bloch- Schneider). Protistology 2006/7;4:353-356.
6
Basu S, Haldar DP. Three new species of Myxobolus Butschli, 1882 from different food fishes of West Bengal, India. Acta Protozool 2003;42:245-251.
7
Basu S, Haldar DP. Description of three new myxosporean species (Myxozoa: Myxosporea: Bivalvulida) of the genera Myxobilatus Davis, 1944 and Myxobolus Butschli, 1882. Acta Protozool 2004;43:337-343.
8
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9
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Seenappa D, Manohar L. Five new species of Myxobolus (Myxosporea, Protozoa), parasitic in Cirrhina mrigala (Hamilton) and Labeo rohita (Hamilton), with a note on a new host record for Myxosporea curmucae Seenappa and Manohar. J Protozool 1981;28:358-360.
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Lalitha-Kumari PS. On a new species of Henneguya (Protozoa: Myxosporidia) from Indian freshwater fish, Ophiocephalus gachua. Riv Parassitol 1965;26:79-84.
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Lalitha-Kumari PS. Studies on parasitic protozoa (Myxosporidia) of freshwater fishes of Andhra Pradesh, India. Riv Parassitol 1969;30:154-225.
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Kaur H, Singh R. Observations on one new species, of genus Myxobolus - M. naini and redescription of M. magauddi recorded from freshwater fishes of Kanjali Wetland of Punjab, India. Proc 20th Nat Cong Parasitology 2008;75-79.
18
Kaur H, Singh R. A new myxosporean species, Myxobolus eirasi sp. nov., a known species M. venkateshi Seenappa, Manohar, 1981 from the Indian major carp fish Cirrhinas mrigala (Ham). Protistology 2009;6:126-130.
19
Kaur H, Singh R. A new myxosporean species Myxobolus sclerii sp. nov., one known species M. stomum Ali et al., 2003 from two Indian major carp fishes. J Parasit Dis 2010;34:33-39.
20
Kaur H, Singh R. One new myxosporidian species, Myxobolus slendrii sp. nov., one known species, M. punjabensis Gupta, Khera, 1989 infecting freshwater fishes in wetlands of Punjab, India. Parasitol Res 2010;106:1043-1047.
21
Kaur H, Singh R. Two new species of Myxobolus (Myxosporea, Bivalvulida) from the Indian major carp Labeo rohita Hamilton, 1822. Protistology 2010;6:264-270.
22
Kaur H, Singh R. Two new species of Myxobolus (Myxozoa: Myxosporea: Bivalvulida) from freshwater fishes of Punjab Wetlands (India). J Parasit Dis 2011; 35:33-41.
23
Kaur H, Singh R. Two new species of Myxobolus (Myxozoa: Myxosporea: Bivalvulida) infecting an Indian major carp in Ropar and Kanjali wetlands (Punjab). J Parasit Dis 2011;35:23-32.
24
Kaur H, Singh R. Myxobolus harikensis sp. nov. (Myxozoa: Myxobolidae) infecting fins of Cirrhina mrigala (Ham.) - An Indian major carp in Harike Wetland, Punjab (India). Parasitol Res 2011;109:1699-1705.
25
Kaur H, Singh R. Two new and one already known species of Myxobolus (Myxozoa:Myxosporea: Bivalvulida infecting gill lamellae of Indian major carp fishes in Ropar and Harike wetlands (Punjab). Proc 22nd Nat Cong Parasitology 2011;200-203.
26
Kaur H, Singh R. Two new species of Myxobolus (Myxozoa: Myxosporea: Bivalvulida) infecting Indian freshwater fishes in Punjab Wetlands (India). Parasitol Res 2011;108:1075-1082.
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Sarkar NK. On two new species of Myxobolus Butschli, 1882 (Myxozoa, Myxosporea) from the fresh-water fishes of West-Bengal, India. Acta Protozool 1986;25:235-239.
38
Seenappa D, Manohar L. Five new species of Myxobolus (Myxosporea, Protozoa), parasitic in Cirrhina mrigala (Hamilton) and Labeo rohita (Hamilton), with a note on a new host record for Myxosporea curmucae Seenappa and Manohar. J Protozool 1981;28:358-3603
39
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40
ORIGINAL_ARTICLE
Promoter hypermethylation of KLOTHO; an anti-senescence related gene in colorectal cancer patients of Kashmir valley
Hypermethylation of CpG islands located in the promoter regions of genes is a major event in the development of the majority of cancer types, due to the subsequent aberrant silencing of important tumor suppressor genes. KLOTHO; a novel gene associated primarily with suppressing senescence has been shown to contribute to tumorigenesis as a result of its impaired function. Recently the relevance of KLOTHO promoter hypermethylation in colorectal carcinoma in humans has been reported. We analyzed the promoter hypermethylation of KLOTHO gene in 50 histopathologically confirmed tumor and adjacent normal tissues of colorectal cancer patients. Methylation was assessed by bisulfite conversion of DNA followed by methylation specific-polymerase chain reaction. Methylation status was compared with gender, smoking status and histopathological parameters of patients. Promoter hypermethylation in KLOTHO gene was detected in 86% (43/50) of tumor tissues and 14% (7/50) of adjacent normal tissues. The methylation pattern differed significantly between tumor and adjacent normal tissues (P<0.0001). However, no association was found between promoter hypermethylation status and gender (P=0.68), smoking status (P=0.64) or other histopathological parameters (P>0.05) of colorectal cancer patients. We conclude that this novel tumor suppressor gene is epigenetically inactivated in colorectal cancer in our population paving way towards the potential of KLOTHO promoter hypermethylation as a predictor of the prognosis in colorectal cancer patients.
https://mbrc.shirazu.ac.ir/article_3231_a7a392e08e513e577af96cfd9b8706b6.pdf
2015-12-01
217
224
10.22099/mbrc.2015.3231
KLOTHO
Promoter hypermethylation
Colorectal cancer
Tumor suppressor
Perveez
Malik
malikp1001@gmail.com
1
Department of General and Minimal Access Surgery, SKIMS Srinagar, India
LEAD_AUTHOR
Ajaz
Malik
ajazamalik@yahoo.com
2
Department of General and Minimal Access Surgery, SKIMS Srinagar, India
AUTHOR
Dil
Afroze
afrozedill@gmail.com
3
Department of Immunology and Molecular Medicine, SKIMS Srinagar, India
AUTHOR
Hamilton, SR, Aalton LA. Pathology and Genetics of Tumours of the Digestive System. IARC Press: Lyon; 2000.
1
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3
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4
Murtaza I, Mushtaq D, Margoob MA, Dutt A, Wani NA, Ahmad I, Bhat ML. A study on P53 alterations in esophageal squamous cell carcinoma & their correlation to common dietary risk factors among population of the Kashmir valley. World J Gastroenterol 2006;12:4033-4037.
5
Javid G, Zargar SA, Rather S, Khan A R, Khan B A,Yattoo GN, Shah A, Gulzar GM, Sodhi JS, Khan MA, Bashir AS. Incidence of CRC in Kashmir valley. Indian J Gastroenterol 2011;30:7-11.
6
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7
Wolf I, Levanon-Cohen S, Bose S, Ligumsky H, Sredni B, Kanety H, Kuro-o M, Karlan B, Kaufman B, Koeffler HP, Rubinek T. Klotho: a tumor suppressor and a modulator of the IGF-1 and FGF pathways in human breast cancer. Oncogene 2008; 27:7094-7105.
8
Pan J, Zhong J, Gan LH, Chen SJ, Jin HC, Wang X, Wang LJ. Klotho, an anti-senescence related gene, is frequently inactivated through promoter hypermethylat-ion in colorectal cancer. Tumour Biol 2011;32:729-735.
9
Wang L, Wang X, Wang X, Jie P, Lu H, Zhang S, Lin X, Lam EK, Cui Y, Yu J, Jin H. Klotho is silenced through promoter hypermethylation in gastric cancer. Am J Cancer Res 2011;1:111-119.
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Lee J, Jeong DJ, Kim J, Lee S, Park JH, Chang B, Jung SI, Yi L, Han Y, Yang Y, Kim KI, Lim JS, Yang I, Jeon S, Bae DH, Kim CJ, Lee MS. The anti-aging gene KLOTHO is a novel target for epigenetic silencing in human cervical carcinoma. Mol Cancer 2010;9:109.
14
Gan LH, Pan J, Chen SJ, Zhong J, Wang LJ. DNA methylation of ZIC1 and KLOTHO gene promoters in colorectal carcinomas and its clinicopathological significance. Zhejiang Da Xue Xue Bao Yi Xue Ban 2011;40:309-314.
15
Xie B, Zhou J, Shu G, Liu DC, Zhou J, Chen J, Yuan L. Restoration of klotho gene expression induces apoptosis and autophagy in gastric cancer cells: tumor suppressive role of Klotho in gastric cancer. Cancer Cell Int 2013;13:18.
16
Wani HA, Bhat AA, Mattoo AA, Khan H, Amin S, Bhat SA, Naikoo NA, Rasheed T, Masood A, Majid S. Distribution of P16 promoter hypermethylation in male/female colorectal cancer patients of Kashmir Valley. Int J Eng Sci Invent 2013;2:11-17.
17
Bird A. Perceptions of epigenetics. Nature 2007;447:396-398.
18
Esteller M. Epigenetics in cancer. N Engl J Med 2008;358:1148-1159.
19
Hoeijmakers JH. DNA damage, aging and cancer. N Engl J Med 2009;361:1475-1485.
20
Collado M, Serrano M. Senescence in tumours: evidence from mice and humans. Nat Rev Cancer 2010;10:51–57.
21
Kuroo M, Matsumura Y, Aizawa H, Kawaguchi H, Suga T, Utsugi T, Ohyama Y, Kurabayashi M, Kaname T, Kume E, Iwasaki H, Iida A, Shiraki-Iida T, Nishikawa S, Nagai R, Nabeshima YI. Mutation of the mouse Klotho gene leads to a syndrome resembling ageing. Nature1997; 390:45–51.
22
Kurosu H, Yamamoto M, Clark JD, Pastor JV, Nandi A, Gurnani P, McGuinness OP, Chikuda H, Yamaguchi M, Kawaguchi H, Shimomura I, Takayama Y, Herz J, Kahn CR, Rosenblatt KP, Kuroo M. Suppression of aging in mice by the hormone Klotho. Science 2005;309:1829-1833.
23
Chan AT, Giovannucci EL. Primary prevention of colorectal cancer. Gastroenterology 2010;138:2029-2043.
24
Wilkins HR, Doucet K, Duke V, Morra A, Johnson N. Estrogen prevents sustained COLO-205 human colon cancer cell growth by inducing apoptosis, decreasing c-myb protein, and decreasing transcription of the anti-apoptotic protein bcl-2. Tumour Biol 2010;31:16-22.
25
Martineti V, Silvestri S, Tonelli F, Brandi ML. Control of colon cancer development and progression by selected estrogen receptor modulators. Expert Rev Endocrinol Metab 2008;3:503-511.
26
Grady WM. Genetic testing for high-risk colon cancer patients. Gastroenterology 2003;124:1574-1594.
27
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28
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29
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30
ORIGINAL_ARTICLE
Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene
The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each in Anzali wetland and Aras reservoir and three rivers Chafrood, Masule Rudkhan and Siah Darvishan. The PCR products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (DdeІ, MboІ, TaqI, RsaІ and HinfІ). Twenty eight composite haplotypes were showed with the number of haplotypes in each population sample ranging from 8 to 13. Private haplotypes were found at very low frequencies. Two regional (Siah Darvishan River and Astara) groups were clearly recognized by cluster and molecular variance model (AMOVA) analyses (P<0.0001). Each of these groups revealed dominant haplotypes while these haplotypes play less important rule in population structures of the other geographic areas. Intrapopulation haplotype (h) and nucleotide (π) diversities were high for each locality, ranging h=0.7560±0.030 and π= 0.00334±0.00301, respectively. Results of this study discerned two genetically divergent populations of narrow-clawed crayfish including Siah Darvishan River and Astara. Thus, the population structure of the narrow-clawed crayfish, as inferred from mtDNA analysis, is constituted by genetically separate groups that nearly reflect their geographic distribution.
https://mbrc.shirazu.ac.ir/article_3265_a1ac3058a017448170346f7a3395ffb2.pdf
2015-12-01
225
237
10.22099/mbrc.2015.3265
Astacus leptodactylus
Mitochondrial DNA
PCR-RFLP
Genetic diversity
Majidreza
Khoshkholgh
majidrezagu@yahoo.com
1
Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmehsara, Iran
AUTHOR
Sajad
Nazari
sajadnazari13@gmail.com
2
Genetic and Breeding Research Center for Coldwater Fishes, Yasouj, Iran
LEAD_AUTHOR
Gherardi F, Souty-Grosset C. Astacus leptodactylus. In: IUCN 2013. IUCN Red List of Threatened Species. Version 2013.2.
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Souty-Grosset C, Reynolds JD. Current ideas on methodological approaches in European crayfish conservation and restocking procedures. Knowl Man Aqua Eco 2009;1:394-395.
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Machino Y, Holdich DM. Distribution of crayfish in Europe and adjacent countries: Updates and comments. Freshwater Cray 2006;15:292-323.
3
Halrioğlu MM, Harlioğlu AG. The harvest of freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823) in Turkey. Rev Fish Biol Fish 2005;14:415-419.
4
Harlioğlu AG, Harlioğlu MM. The status of freshwater crayfish (Astacus leptodactylus Eschscholtz) fisheries in Turkey. Rev Fish Sci2009;17:187-189.
5
Holdich DM. A review of astaciculture: freshwater crayfish farming. Aquat Livin Res 1993;6:307-317.
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Veladykov VD. Inland fisheries resources of Iran especially of the Caspian Sea with special reference to Sturgeon, Report to Government of Iran, FAO Report, FAO, 1964; Rome, 188, 64 p.
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Avise JC. Molecular Markers, Natural History, and Evolution. Chapman and Hall 1994, New York, NY. 511 pp.
8
Frankham R. Relationship of genetic variation to population size in wildlife. Conserv Biol 1996;10:1500
9
Ludwig A, Kirschbaum F. Comparison of mitochondrial DNA sequences between the European and the Adriatic sturgeon. J Fish Biol 1998;52:1289-1291.
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Billington N. Mitochondrial DNA. Pages 59-100 in EM. Hallerman, editor. Population genetics: principles and applications for fisheries scientists. American Fisheries Society, 2003: Bethesda, Maryland.
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Khoshkholgh M, NazariS, Pourkazemi M. Population structure of Persian sturgeon (Acipenser persicus Borodin, 1897) populations in the southern part of Caspian Sea. Iran J Anim Bio 2013;9:29-39.
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13
Pourkazemi M, Nazari S, Khoshkholgh MR, Azizzadeh L. Genetic relationships among populations of the Persian sturgeon, Acipenser percicus, in the south Caspian Sea detected by mitochondrial DNA–restriction fragment length polymorphisms. Caspian J Env Sci 2012;10:215-226.
14
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15
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