Shiraz University Press
Molecular Biology Research Communications
2322-181X
2345-2005
2
3
2013
07
01
Synthesis and coating of nanosilver by vanillic acid and its effects on Dunaliella salina Teod.
47
55
EN
Hajar
Zamani
Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran
hzamani@shirazu.ac.ir
Ali
Moradshahi
Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran
moradshahi@susc.ac.ir
10.22099/mbrc.2013.1571
Plant phenolics have high reducing capacity which can be exploited in the synthesis of nanomaterials. In the present study, phytoreductant vanillic acid is used to produce and coat silver nanoparticles. The effects of Ag nanoparticles on the unicellular green algae <em>D. Salina</em> were then investigated. Under optimum pH and temperature, silver ions were reduced to silver metal by vanillic acid. The absorption spectra of the silver nanoparticles showed a maximum band of 410 nm, which is characteristic of the surface plasmon resonance of silver nanoparticles. Dynamic light scattering (DLS) showed a narrow distribution size with an average of 52 nm. High concentrations of Ag nanoparticles reduced growth, total carotenoids, chlorophyll content, phenolics and antioxidant activity of the algae. Based on these results, phytoreductant vanillic acid can be used for synthesis and coating of nanosilver. Due to the projected increase in quantities and types of nanomaterials which leads to their elevated release into the environment and also because of the toxicity of nanomaterials, an urgent need to evaluate the impacts of nano-sized particles on the environment and living organisms is felt.
Green chemistry,Phenolic compounds,Ag nanoparticles,Dunaliella salina
https://mbrc.shirazu.ac.ir/article_1571.html
https://mbrc.shirazu.ac.ir/article_1571_0fa39d8ff54ade66116eec1b301bc86b.pdf
Shiraz University Press
Molecular Biology Research Communications
2322-181X
2345-2005
2
3
2013
07
01
A convenient method to generate methylated and un-methylated control DNA in methylation studies
57
61
EN
Mehdi
Manoochehri
Biotechnology department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
mhd.manoochehri@yahoo.com
Mojgan
Bandehpour
Biotechnology department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
bandehpour@gmail.com
Bahram
Kazemi
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
bahram_14@yahoo.com
10.22099/mbrc.2013.1647
Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole genome amplification and enzymatic methylation were performed to generate un-methylated and methylated DNA. The generated DNA were confirmed using methylation sensitive/dependant enzymes, and methylation specific PCR. Control reaction assays confirmed the generated methylated and un-methylated DNA.
Epigenetics,DNA methylation,Control DNA
https://mbrc.shirazu.ac.ir/article_1647.html
https://mbrc.shirazu.ac.ir/article_1647_e89abf4aa7e2227127b874a1f07dcd95.pdf
Shiraz University Press
Molecular Biology Research Communications
2322-181X
2345-2005
2
3
2013
07
01
Isolation of Brassica napus MYC2 gene and analysis of its expression in response to water deficit stress
63
71
EN
Massumeh
Aliakbari
Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran
massume.aliakbari@gmail.com
Hooman
Razi
Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University
razi@shirazu.ac.ir
10.22099/mbrc.2013.1648
Manipulation of stress related transcription factors to improve plant stress tolerance is a major goal of current biotechnology researches. <em>MYC2</em> gene encodes a key stress-related transcription factor involved in Jasmonate (JA) and abscisic acid (ABA) signaling pathways in Arabidopsis. <em>Brassica napus</em>, as a globally important oilseed crop, is a close relative of Arabidopsis. In the present study, a 960bp cDNA fragment of <em>B. napus</em> <em>MYC2</em> (<em>BnMYC2</em>) was isolated, cloned and sequenced. The deduced amino acid sequence of the <em>BnMYC2</em> cDNA fragment showed high homology with <em>Arabidopsis thaliana</em> <em>MYC2</em> and the putative <em>Brassica oleracea</em> <em>MYC2</em>, implying the conserved functions among these orthologous genes. The expression analysis by a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that <em>BnMYC2</em> is a drought inducible gene. A different expression profile of <em>BnMYC2</em> was observed between drought tolerant and sensitive <em>B. napus</em> cultivars. The drought tolerant cultivar showed a higher accumulation of <em>BnMYC2</em> transcript in response to water deficit stress during the studied time course. This result indicates that <em>BnMYC2</em> may contribute to drought tolerance in <em>B. napus</em>.
Brassica napus,MYC2 transcription factor,Semi-quantitative RT-PCR,Water deficit stress
https://mbrc.shirazu.ac.ir/article_1648.html
https://mbrc.shirazu.ac.ir/article_1648_b60301af29ec5cd65ab91997cfa6e449.pdf
Shiraz University Press
Molecular Biology Research Communications
2322-181X
2345-2005
2
3
2013
07
01
Growth and pigment development of Dunaliella salina Teod. in response to ammonium nitrate nutrition
73
79
EN
Keramatollah
Nikookar
Biology Department, Collage of Sciences, Shiraz University, Shiraz 71454, I. R. Iran
nikookar@susc.ac.ir
Lahya
Rowhani
Biology Department, Collage of Sciences, Shiraz University, Shiraz 71454, I. R. Iran
Sasan
Mohsenzadeh
0000000342904529
Biology Department, Collage of Sciences, Shiraz University, Shiraz 71454, I. R. Iran
mohsenz@shirazu.ac.ir
Bahman
Kholdebarin
Biology Department, Collage of Sciences, Shiraz University, Shiraz 71454, I. R. Iran
bkholdeb@susc.ac.ir
10.22099/mbrc.2013.1649
The microalgae, <em>Dunaliella salina</em> was isolated from Maharlu Salt Lake, south east of Shiraz, Iran. The isolated strain was identified by both morphological and physiological markers. The complete ITS region (ITS1 + ITS2) including the 5.8S rDNA gene used as molecular marker confirmed our identification. Growth and cell proliferation, total chlorophyll and carotenoid contents were determined in the presence of 0.125, 0.25, 0.50, 1.0 and 2.0 mM ammonium nitrate. After five weeks, a maximum cell density of about (4.4 ±0.21)×10<sup>6 </sup>mL<sup>-1</sup> was observed in the growth medium containing 1mM NH<sub>4</sub>NO<sub>3</sub>. Increasing NH<sub>4</sub>NO<sub>3 </sub>concentrations up to 1mM, resulted in an increase in the cells total chlorophyll contents. The highest amount of cell carotenoid contents was produced in media containing the least amount of NH<sub>4</sub>NO<sub>3</sub> (0.125 mM).<sub> </sub>Manipulating the type and amount of external nitrogen sources to induce the synthesis of the highest amounts of carotenoid compounds in this microalgae strain can be of great commercial values to food industries.
Dunaliella salina,ITS,ammonium nitrate,Carotenoid synthesis
https://mbrc.shirazu.ac.ir/article_1649.html
https://mbrc.shirazu.ac.ir/article_1649_4bcad9bfc002e6b2724a438dbc3aab6e.pdf
Shiraz University Press
Molecular Biology Research Communications
2322-181X
2345-2005
2
3
2013
07
01
Genetic variation of Garra rufa fish in Kermanshah and Bushehr provinces, Iran, using SSR microsatellite markers
81
88
EN
Ali
Shabani
Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran
shilat86_iut@gmail.com
Ghasem
Askari
Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran
askarighasem82@gmail.com
Amin
Moradi
Marine Sciences and Technology of Khoramshahr University
moradi.1986@yahoo.com
10.22099/mbrc.2013.1665
Six highly variable microsatellite loci were used to investigate the genetic diversity and population structure of the <em>Garra rufa</em> in Kermanshah and Bushehr provinces, Iran. All of the 6 microsatellite loci screened in this study showed polymorphism. A total of 90 individual fish from 3 populations were genotyped and 60 alleles were observed in all loci. The number of alleles per locus ranged from 6 to14. The average allelic number of these polymorphic markers was 10. The averages of observed (Ho) and expected heterozygosity (He) was 0.529 and 0.826, respectively. The genetic distance values ranged between 0.235-0.570. The UPGMA dendrogram based on genetic distance resulted in three clusters: Gamasiab population alone was classified as one and the other two populations as the second cluster. This study revealed a fairly high level of genetic variation in the microsatellite loci within the three populations, and identified distinct population groups of <em>Garra rufa</em>. This study gains significance for the analysis of the populations’ genetic diversity as well as the management of this important fish resource.
Garra rufa,Genetic diversity,Population,Kermanshah,Bushehr
https://mbrc.shirazu.ac.ir/article_1665.html
https://mbrc.shirazu.ac.ir/article_1665_40d4207b64460123c119564d05b068c9.pdf
Shiraz University Press
Molecular Biology Research Communications
2322-181X
2345-2005
2
3
2013
07
01
Reduction of NADH oxidase, NO synthase, TNFα, and IL-1β mRNA expression levels on lipopolysacharide-stimulated murine macrophages by Zataria Multiflora
89
100
EN
Parastoo
Karimian
Zataria multiflora and anti-inflammatory effects
parastookarimian1@yahoo.com
Gholamreza
Kavoosi
Institute of Biotechnology , Shiraz University, Shiraz, Iran
ghkavoosi@shirazu.ac.ir
Zahra
Amieghofran
Department of Immunology, Autoimmune Disease Research Center and Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
zamirghof@sums.ac.ir
Fatholla
Kalantar
Department of Immunology, Autoimmune Disease Research Center and Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
kalantar@yahoo.com
10.22099/mbrc.2013.1726
Zataria multiflora (ZM) is a thyme-like aromatic plant in the Lamiaceae family that grows in central and southern Iran. ZM is extensively used as a flavor ingredient in a wide variety of foods and is used as part of popular traditional folk remedies. In the present study, ZM essential oil (ZMO) was obtained from ZM leaves via hydro-distillation and then analyzed by GC-MS (gas chromatography-mass spectrometry). The anti-inflammatory activity of ZMO was determined via measures of NADH oxidase (NOX), inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, and interleukin (IL)-1β mRNA expression in lipopolysaccharide-stimulated murine macrophages using real-time polymerase chain reaction (PCR). GC-MS analysis indicated that the main components in the ZMO were carvacrol (29.4%), thymol (25.7%), p-cymene (11.2%), linalool (9.3%), and γ-terpinene (8.0%). ZMO significantly reduced NOX, iNOS, TNFα, and IL-1β mRNA expression in cells at concentrations of 0.1-1 μg/mL, indicating a capacity for this product to potentially modulate/diminish immune responses. ZMO has anti-oxidant and anti-inflammatory properties and could be potentially used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and a number of inflammatory conditions associated with stress.
Macrophages,Zataria multiflora,NADH Oxidase,NO Synthase,TNFα,and IL-1β
https://mbrc.shirazu.ac.ir/article_1726.html
https://mbrc.shirazu.ac.ir/article_1726_cb111b4e8779a871ede3ec2744276557.pdf