Shiraz University Press Molecular Biology Research Communications 2322-181X 4 2 2015 06 01 Isolation and molecular characterization of partial FSH and LH receptor genes in Arabian camels (Camelus dromedarius) 105 114 3027 10.22099/mbrc.2015.3027 EN Saber Jelokhani-Niaraki department of animal science, faculty of agriculture, Ferdowsi University of Mashhad Mojtaba Tahmoorespur department of animal science, faculty of agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Morteza Bitaraf-Sani department of animal science, faculty of agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Journal Article 2015 02 14 Very little is known about LHR and FSHR genes of domestic dromedary camels. The main objective of this study was to determine and analyze partial genomic regions of FSHR and LHR genes in dromedary camels for the first time. To this end, a total of 50 DNA samples belonging to dromedary camels raised in Iran were sent for sequencing (25 samples of each gene). We compared the nucleotide sequences of Camelus dromedarius with corresponding sequences of previously published FSHR and LHR genes in bactrian camels and other species. According to the data, the same nucleotide variation was identified in both regions of the two camel species. The alignment of deduced protein sequences of the two different species revealed an amino acid variation at the FSHR region. No evidence of amino acid variation was observed, however, in LHR sequences. Phylogenetic analysis indicated that both camel species had a close relationship and clustered together in a separate branch. This was further confirmed by genetic distance values illustrating significant sequence identity between Camelus dromedarius and Camelus bactrianus. Interestingly, sequence comparisons revealed heterozygote patterns in FSHR sequences isolated from dromedary camels of Iran. In comparison to other species, this camel contains three amino acid substitutions at 5, 67, and 105 positions in the FSHR coding region. These positions are found exclusively in camels and can be considered as species specific. The results of our study can be used for hormone functionality research (FSHR and LHR) as well as reproduction-linked polymorphisms and breeding programs.

https://mbrc.shirazu.ac.ir/article_3027_f6b9e53f8ddca78c36e0260a497b4df5.pdf