Shiraz University Press Molecular Biology Research Communications 2322-181X 15 2 2026 06 01 Therapeutic potential of 4-OH-coumarin in neuroblastoma: cellular, molecular, and epigenetic insights 103 116 8512 10.22099/mbrc.2025.54532.2228 EN Çağrı Öner Department of Medical Biology, Medical Faculty, Kırklareli University, Kırklareli, Türkiye Department of Medical Biology and Genetics, Medical Faculty, Maltepe University, İstanbul, Türkiye Halise Elif Kulakac Medical Faculty, Maltepe University, İstanbul, Türkiye Damla Kolcuoglu Department of Medical Biology, Medical Faculty, Kırklareli University, Kırklareli, Türkiye Ertuğrul Çolak Department of Biostatistics, Medical Faculty, Eskişehir Osmangazi University, Eskişehir, Türkiye Journal Article 2025 10 13 The effect of 4-OH-Coumarin, a warfarin derivate, on the cellular characteristics and metastasis of SH-SY5Y neuroblastoma cells and HUVEC cells was aimed to determine. After IC50 concentrations were detected wound healing and hematoxylin-eosin assays were performed on both cell lines. Ki-67, hTERT, PI3K, AKT, mTOR, HIF-1α, PINK1, Parkin, Cyt C, p53 gene expressions and piR-651, piR-823, miR-126 expressions were determined by RT-PCR. The proliferation, wound closure and survival decreased after 4-OH-Coumarin treatment (<em>p</em><0.001). Ki-67, hTERT, PI3K, mTOR, HIF-1α, PINK1, Parkin, Cyt C and piR-823 expressions were decreased, while AKT, p53, piR-651, and miR-126 increased on SH-SY5Y (<em>p</em><0.001). AKT (<em>p</em><0.05), Parkin, and piR-651 expressions increased on only HUVEC cells (p<0.001). We believe that the study of various molecules that are secondary metabolites such as 4-OH-Coumarin may provide valuable data to observe the effects and mechanisms of new therapeutics that have the potential to be used for cancer treatment. https://mbrc.shirazu.ac.ir/article_8512_5f87f1462ab40b514f020fbb46695a45.pdf