Shiraz University PressMolecular Biology Research Communications2322-181X12420231201Genetic association between rs1695 in glutathione S-transferase P1 and risk of periodontitis: a pilot study133137720410.22099/mbrc.2023.46999.1815ENRakshithaVettri SaravananDepartment of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77AnithaPandiCentre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77KarthikeyanMurthykumarDepartment of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77SmilineGirija Aseervatham SelviDepartment of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77ParamasivamArumugamCentre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77VijayashreePriyadharsini JayaseelanCentre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77Journal Article20230315The present study aims to determine the association between a genetic polymorphism of <em>GSTP1</em> (rs1695) and the risk of periodontitis. This study used a cross-sectional design and included subjects from the South Indian population. A total of 100 individuals enrolled at Saveetha Dental College and Hospital, Tamil Nadu were included in this study. The participants were divided into control (n=50) and periodontitis (n=50) based on clinical examination. Blood samples were collected. Genotyping was performed using specific primers spanning the polymorphic site. The genotypic frequencies for the rs1695 polymorphism were not significantly different between cases and controls.https://mbrc.shirazu.ac.ir/article_7204_131a2179c3a8b5ab6b08789fd6f7b3b7.pdfShiraz University PressMolecular Biology Research Communications2322-181X12420231201Сytotoxic effect of CAR-T cells against modified MCF-7 breast cancer cell line139148720510.22099/mbrc.2023.47125.1820ENAigul Kh.ValiullinaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaEkaterina A.ZmievskayaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaIrina A.GaneevaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia0000-0003-4041-5000Margarita N.ZhuravlevaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaEkaterina E.GaraninaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaAlbert A.RizvanovInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaAlexey V.PetukhovInstitute of Hematology, Almazov National Medical Research Center, 197341 Saint Petersburg, RussiaEmil R.BulatovInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaJournal Article20230410The most often diagnosed and fatal malignancy in women is breast cancer. The International Agency for Research on Cancer (IARC) estimates that there are 2.26 million new cases of cancer in 2020. Adoptive cell therapy using T cells with chimeric antigen receptor shows potential for the treatment of solid tumors, such as breast cancer. In this work the effectiveness of CAR-T cells against monolayer and three-dimensional bioprinted tumor-like structures made of modified MCF-7 breast cancer cells was assessed. The cytokine profile of supernatants after co-cultivation of MCF-7 tumor cell models with CAR-T cells was also measured to reveal the inflammatory background associated with this interaction.https://mbrc.shirazu.ac.ir/article_7205_0769792c94b40b0c5d397e7a69ab09bf.pdfShiraz University PressMolecular Biology Research Communications2322-181X12420231201Human BKV large T genome detection in prostate cancer and benign prostatic hyperplasia tissue samples by nested PCR: A case-control study149154720610.22099/mbrc.2023.47537.1836ENNargesTavassoliDepartment of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranArastooVojdaniDepartment of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranSaraSalimi-NaminDepartment of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0009-0005-1966-6878MajidKhadem-RezaiyanDepartment of Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMahmoudrezaKalantariDepartment of Pathology, Mashhad Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMasoudYoussefiDepartment of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranAntimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0003-0534-5393Journal Article20230601Human BK polyomavirus (BKPyV) is a latent infectious agent in the genitourinary tract associated with hemorrhagic cystitis and nephropathy. This virus can be a risk factor for various human malignancies, including prostate cancer (PCa). It may contribute to prostate cancer development, as it demonstrates oncogenic properties by encoding oncoproteins. This study assessed the prevalence of this virus in benign and malignant prostate tissues. Between 2009 and 2019, 49 formalin-fixed paraffin-embedded (FFPE) PCa and 49 benign prostatic hyperplasia (BPH) samples were gathered from the pathology department of a tertiary care university hospital. They were used as cases and controls, respectively. After deparaffinization and DNA extraction, nested PCR was applied to identify the BKPyVgp5 gene (LTAg) using inner and outer primers. The nested PCR showed a 278-bp bond corresponding to the BKPyVgp5 genome (LTAg) in 53.1% (26/49) of PCa and 14.3% (7/49) of BPH (p<0.001). The presence of BKV was significantly associated with an increased risk of PCa development (OR=6.78, 95% CI=2.55–18.02, p<0.001). The BKV LTAg gene was significantly more prevalent in PCa samples than in BPH samples. These results demonstrate the presence of the virus in prostate cancer tissues.https://mbrc.shirazu.ac.ir/article_7206_4c0d7640e68116f0bac4418baf08df17.pdfShiraz University PressMolecular Biology Research Communications2322-181X12420231201A comparison of phylogenetic and distance-based approaches for the distinction of genetically closed species, Draba rimarum (Rech.f.) A.R. Khosravi & A. Eslami-Farouji, and Draba aucheri Boiss. (Brassicaceae) as a case study155163720710.22099/mbrc.2023.47706.1842ENAtenaEslami-FaroujiDepartment of Biology, School of Science, Shiraz University, Shiraz, IranAhmad RezaKhosraviDepartment of Biology, School of Science, Shiraz University, Shiraz, IranMehdiGholamiDepartment of Biology, School of Science, Shiraz University, Shiraz, IranSasanMohsenzadehDepartment of Biology, School of Science, Shiraz University, Shiraz, IranJournal Article20230623Circumscribing species boundries is necessary in systematic plant biology. Even a mistake in delimiting taxa may lead to incorrect scientific interpretations. <em>Draba rimarum </em>(Rech.f.) A.R. Khosravi & A. Eslami-Farouji is an endemic Iranian species with a narrow geographic distribution, and is genetically close to <em>D. aucheri</em>. The present study provided a phylogenetic review, time divergence, and planar network of both species to unravel the distinct position of both species along with the prediction of any conflicting or ambiguous signals. Regarding this purpose, here we represent that phylogenetic trees may fail to show reliable results toward the distinct position of genetically close species.https://mbrc.shirazu.ac.ir/article_7207_0dc0341a3539b1f302f5b130f5d0fd25.pdf