Shiraz University PressMolecular Biology Research Communications2322-181X15220260601A strategic approach to genetic model selection in association studies: A practical guide8183849510.22099/mbrc.2026.54476.2226ENMostafaSaadatDepartment of Biology, School of Science, Shiraz University, Shiraz 71467-13565, IranJournal Article20251008No abstracthttps://mbrc.shirazu.ac.ir/article_8495_1a4efc0950f9aa60dc4dd721509052b3.pdfShiraz University PressMolecular Biology Research Communications2322-181X15220260601A systematic review on the potential of modulating the IRE arm of the UPR in U87 and U251 glioblastoma cells for improved therapeutic efficacy85101849910.22099/mbrc.2025.54152.2205ENMehdiGhavamizadehAutophagy Research Center, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranRoozbehKianiAutophagy Research Center, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranFatemehShamsStudent Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranEndocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranParmisTaghizadehAutophagy Research Center, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranAliHonari-JahromiAutophagy Research Center, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranSeyed Mohammad HosseinAfzali-JoibariNanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranMozhdehZamaniAutophagy Research Center, Shiraz University of Medical Sciences, Shiraz, IranSanazDastghaibEndocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranPoonehMokarramAutophagy Research Center, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, IranJournal Article20250902Glioblastoma multiforme (GBM) remains the most aggressive primary brain tumor with poor prognosis and limited response to current therapies. Recent studies suggest that the unfolded protein response (UPR), particularly the inositol-requiring enzyme 1 (IRE1) signaling arm, plays a pivotal role in GBM pathophysiology by mediating cellular adaptation to endoplasmic reticulum stress. This systematic review evaluates the role of IRE1 in GBM cell lines (U87, U251) and investigates whether its activation or inhibition affects migration, proliferation, apoptosis, and cell death.<strong> </strong>A comprehensive search was conducted on PubMed/ Medline, Scopus, Web of Science, and Embase using various keywords up to October 14, 2025, following the PRISMA guidelines. The search aimed to identify original English-language studies that specifically examined and analyzed the IRE1 arm of the UPR pathway in glioblastoma cells. Out of 466 records, 26 studies met the inclusion criteria. Twenty studies explored IRE1 activation, while six investigated its inhibition. IRE1 activation yielded dual effects—promoting apoptosis via JNK or XBP1 pathways in some contexts, while supporting tumor survival and angiogenesis through XBP1-mediated transcription and RIDD suppression in others. Dual role of IRE1 could sensitize GBM cells to chemotherapy agents, reduced migration and proliferation, and induced apoptosis. IRE1 acts as a context-dependent regulator in GBM, showing both pro-survival and pro-death roles. Most studies report that IRE1 activation promotes glioblastoma cell death, while fewer address its inhibition. Thus, both activation and inhibition may offer therapeutic potential depending on cellular context and downstream signaling<em>.</em>https://mbrc.shirazu.ac.ir/article_8499_f4a809b18deb5ea23e2891724eef2de2.pdfShiraz University PressMolecular Biology Research Communications2322-181X15220260601Therapeutic potential of 4-OH-coumarin in neuroblastoma: cellular, molecular, and epigenetic insights103116851210.22099/mbrc.2025.54532.2228ENÇağrıÖnerDepartment of Medical Biology, Medical Faculty, Kırklareli University, Kırklareli, TürkiyeDepartment of Medical Biology and Genetics, Medical Faculty, Maltepe University, İstanbul, TürkiyeHalise ElifKulakacMedical Faculty, Maltepe University, İstanbul, TürkiyeDamlaKolcuogluDepartment of Medical Biology, Medical Faculty, Kırklareli University, Kırklareli, TürkiyeErtuğrulÇolakDepartment of Biostatistics, Medical Faculty, Eskişehir Osmangazi University, Eskişehir, TürkiyeJournal Article20251013The effect of 4-OH-Coumarin, a warfarin derivate, on the cellular characteristics and metastasis of SH-SY5Y neuroblastoma cells and HUVEC cells was aimed to determine. After IC50 concentrations were detected wound healing and hematoxylin-eosin assays were performed on both cell lines. Ki-67, hTERT, PI3K, AKT, mTOR, HIF-1α, PINK1, Parkin, Cyt C, p53 gene expressions and piR-651, piR-823, miR-126 expressions were determined by RT-PCR. The proliferation, wound closure and survival decreased after 4-OH-Coumarin treatment (<em>p</em><0.001). Ki-67, hTERT, PI3K, mTOR, HIF-1α, PINK1, Parkin, Cyt C and piR-823 expressions were decreased, while AKT, p53, piR-651, and miR-126 increased on SH-SY5Y (<em>p</em><0.001). AKT (<em>p</em><0.05), Parkin, and piR-651 expressions increased on only HUVEC cells (p<0.001). We believe that the study of various molecules that are secondary metabolites such as 4-OH-Coumarin may provide valuable data to observe the effects and mechanisms of new therapeutics that have the potential to be used for cancer treatment.https://mbrc.shirazu.ac.ir/article_8512_5f87f1462ab40b514f020fbb46695a45.pdfShiraz University PressMolecular Biology Research Communications2322-181X15220260601Molecular and Morphological evidence support the delimitation of a new genus, Hafezia gen. nov. in Arabideae (Brassicaceae)117130851510.22099/mbrc.2025.54085.2198ENAhmad RezaKhosraviDepartment of Biology, College of Science, Shiraz University, Shiraz, IranAtenaEslami-FaroujiDepartment of Biology, College of Science, Shiraz University, Shiraz, IranJournal Article20250908Recent molecular phylogenetic analyses within the tribe Arabideae (Brassicaceae) have uncovered unresolved lineages that challenge current generic boundaries. In this study, we incorporate molecular data with morphological evidence to resolve a long-standing taxonomic ambiguity, resulting in the description of a new monophyletic genus, <em>Hafezia</em> gen. nov., together with two new combinations, <em>H. aucheri </em>(Boiss.) A.R. Khosravi & A. Eslami-Farouji<em> </em>and <em>H. parvula </em>(Dufour ex DC.) A.R. Khosravi & A. Eslami-Farouji<em>. </em>Integrative taxonomy and phylogenetic insights, such as indels and substitution nucleotide variants, confirm the distinct assignment of these taxa, previously misassigned to <em>Arabis</em> L. Morphological reassessment, including traits such as simple, long setaceous hairs, notched petals, winged stamens, not compressed, not subtorulose or torulose fruits, supports their separation. The new genus naturally grows in the Mediterranean and west of the Irano-Turanian floristic regions. Our findings highlight the significance of integration of taxonomic investigations with the molecular investigations in clarifying evolutionary relationships and provide a revised key for accurate identification within Arabideae.https://mbrc.shirazu.ac.ir/article_8515_991bc9ff9ae45909c50ec5f977388867.pdfShiraz University PressMolecular Biology Research Communications2322-181X15220260601Observations and points of interest regarding bioinformatics predictions of miRNA targeting syncytin genes in endometriosis and miscarriage131132856510.22099/mbrc.2025.55165.2254ENPiruzShadbashBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranMarziehBahari-BabadiDepartment of Biochemistry, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranJournal Article20251210No abstracthttps://mbrc.shirazu.ac.ir/article_8565_1dc0bb48f080d40ee40c5aade850ce2e.pdfShiraz University PressMolecular Biology Research Communications2322-181X15220260601Response to the Letter: Observations and points of interest regarding bioinformatics predictions of miRNA targeting syncytin genes in endometriosis and miscarriage133133856610.22099/mbrc.2025.55184.2256ENSomayehShatizadeh-MalekshahiDepartment of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranJournal Article20251213No abstracthttps://mbrc.shirazu.ac.ir/article_8566_8a867b7fbd6edb3cb1450890d7ae0544.pdf