Shiraz University Press Molecular Biology Research Communications 2322-181X 4 4 2015 12 01 The binding assessment with human serum albumin of novel six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands 167 179 3165 10.22099/mbrc.2015.3165 EN Reza Yousefi Protein Chemistry Laboratory (PCL), Department of Biology, Shiraz University, Shiraz, Iran. Institute of Biotechnology, Shiraz University, Shiraz, Iran Asghar Taheri-Kafrani Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran Sayed Masoud Nabavizadeh Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran Zahra Pouryasin Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran Mohammad Bagher Shahsavani Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran Kazem Khoshaman Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran Mehdi Rashidi Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran Journal Article 2015 05 25 The interactions between platinum complexes and human serum albumin (HSA) play crucial roles in the distribution, metabolism, and activity of platinum-based anticancer drugs. Octahedral platinum (IV) complexes represent a significant class of anticancer agents that display molecular pharmacological properties different from cisplatin. In this study, the interaction between two Pt(IV) complexes with the general formula [Pt(X)2Me2 (tbu2bpy)], where tbu2bpy = 4,4′-ditert-butyl-2,2′-bipyridine, with two leaving groups of X = Cl (Com1) or Br (Com2), and HSA were investigated, using Ultraviolet-Visible (UV-Vis) spectroscopy, fluorescence spectroscopy, circular dichroism (CD) and molecular docking simulation. The spectroscopic and thermodynamic data revealed that the HSA/Pt(IV) complexes interactions were spontaneous process and Com2 demonstrated stronger interaction and binding constant in comparison with Com1. Also, the results suggest approximately similar structural alteration of HSA in the presence of these Pt complexes. Molecular docking revealed that both Pt(IV) complexes bind with HSA in subdomain IB, literally the same as each other. This study suggests that variation in the leaving group, displaying differing departure rate, has no significant contribution in denaturing prosperities of the Pt(IV) complexes against HSA.

https://mbrc.shirazu.ac.ir/article_3165_ecc2d55b361bb567ec45b604cd1fd6ba.pdf

Shiraz University Press Molecular Biology Research Communications 2322-181X 4 4 2015 12 01 Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii) 181 188 3166 10.22099/mbrc.2015.3166 EN Zeinab Abdolahnejad Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran. Mohammad Pourkazemi Iran Fishery Research Organization (IFRO), Tehran, Iran. Majid Reza Khoshkholgh Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran. Mahtab Yarmohammadi International Sturgeon Research Institute, Rasht-Iran. Journal Article 2015 05 27 The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon.

https://mbrc.shirazu.ac.ir/article_3166_004ad7f4d0e422dda0d9ed4b9d3af5a1.pdf

Shiraz University Press Molecular Biology Research Communications 2322-181X 4 4 2015 12 01 Molecular systematics and distribution review of the endemic cyprinid species, Persian chub, Acanthobrama persidis (Coad, 1981) in Southern Iran (Teleostei: Cyprinidae) 189 206 3173 10.22099/mbrc.2015.3173 EN Azad Teimori Kerman University Hamid Reza Esmaeili Department of Biology, College of Sciences, Shiraz University Golnaz Sayyadzadeh Shiraz University Neda Zarei Shiraz University Ali Gholamhosseini Shiraz University Journal Article 2015 05 15 The Iranian Persian chub is an endemic species of the family Cyprinidae known only from few localities in drainages of Southern Iran. It was originally described in the genus Pseudophoxinus as (Pseudophoxinus persidis) and then Petroleuciscus (as Petroleuciscus persidis). In this study, we examined phylogenetic relationships of the Iranian Persian chubwith other relatives in the family Cyprinidae based on the mitochondrial cytochrome b gene to estimate the phylogenetic (and taxonomic) position of the species. Our molecular phylogenies show that new fish sequences from the drainages in southern Iran are clustered with sequences of the genus Acanthobrama from GenBank while the sequences from two other genera (Pseudophoxinus and Petroleuciscus) are in distinct clade. Therefore, we conclude that the populations of Persian Chub in drainages of southern Iran (i.e., Kol, Kor, Maharlu and Persis) belong to the genus Acanthobrama andspecies Acanthobrama persidis. The predicted geographic distributions for the species showed a large area of suitable climate for A. persidis across south and west of Iran especially in the Kor River basin. Some other parts in the Persis and Tigris are also might have been suitable habitats for this cyprinid species showing possible dispersal route of Acanthobrama from Tigris to the Persis, Kor and Kol basins.

https://mbrc.shirazu.ac.ir/article_3173_0a41e187c5794418cbd45cd917a5fb43.pdf

Shiraz University Press Molecular Biology Research Communications 2322-181X 4 4 2015 12 01 Description of a myxozoan parasite Myxobolus sp.n PKB2014 from an edible fish, with emphasis on its molecular characterization 207 216 3179 10.22099/mbrc.2015.3179 EN Somerita Panda University of Kalyani Subarna Ghosh University of Kalyani Probir Kumar Bandyopadhyay UNIVERSITY OF KALYANI Journal Article 2015 02 18 In India, more than 104 species of Myxobolus have so far been reported infecting freshwater as well as marine fishes. The study focuses on the description of a new myxosporean species, Myxobolus sp.n PKB 2014 from the gill lamellae of an Indian major carp Labeo rohita. The species have been described on the basis of morphological characterization of the spores, tissue architecture and 18S rDNA sequence data. The plasmodia of Myxobolus sp.n PKB 2014 were round in shape measuring, 50 to 70 μm in diameter and spores were ellipsoidal in frontal view measures 14.7 ± 0.51 μm. The 18S rRNA nucleotide sequence with 806 bp of Myxobolus sp.n PKB 2014 (Accession number KJ652226) clustered phylogenitically with other Myxobolus spp. infecting cyprinid gills with 90-99% similarity. According to the phylogenetic study we concluded that M. wulli was the closest relative having 99% similarity with the species under description but the sequence was distinct in each species which additionally exhibited different morphological features. The infection rate was low to moderate. After through comparison it can be concluded that the species being described here is new to science which is designated as Myxobolus sp.n.PKB 2014.

https://mbrc.shirazu.ac.ir/article_3179_028448edd3b98239227dfaa8daaa6332.pdf

Shiraz University Press Molecular Biology Research Communications 2322-181X 4 4 2015 12 01 Promoter hypermethylation of KLOTHO; an anti-senescence related gene in colorectal cancer patients of Kashmir valley 217 224 3231 10.22099/mbrc.2015.3231 EN Perveez Malik Department of General and Minimal Access Surgery, SKIMS Srinagar, India Ajaz Malik Department of General and Minimal Access Surgery, SKIMS Srinagar, India Dil Afroze Department of Immunology and Molecular Medicine, SKIMS Srinagar, India Journal Article 2015 07 30 Hypermethylation of CpG islands located in the promoter regions of genes is a major event in the development of the majority of cancer types, due to the subsequent aberrant silencing of important tumor suppressor genes. KLOTHO; a novel gene associated primarily with suppressing senescence has been shown to contribute to tumorigenesis as a result of its impaired function. Recently the relevance of KLOTHO promoter hypermethylation in colorectal carcinoma in humans has been reported. We analyzed the promoter hypermethylation of KLOTHO gene in 50 histopathologically confirmed tumor and adjacent normal tissues of colorectal cancer patients. Methylation was assessed by bisulfite conversion of DNA followed by methylation specific-polymerase chain reaction. Methylation status was compared with gender, smoking status and histopathological parameters of patients. Promoter hypermethylation in KLOTHO gene was detected in 86% (43/50) of tumor tissues and 14% (7/50) of adjacent normal tissues. The methylation pattern differed significantly between tumor and adjacent normal tissues (P<0.0001). However, no association was found between promoter hypermethylation status and gender (P=0.68), smoking status (P=0.64) or other histopathological parameters (P>0.05) of colorectal cancer patients. We conclude that this novel tumor suppressor gene is epigenetically inactivated in colorectal cancer in our population paving way towards the potential of KLOTHO promoter hypermethylation as a predictor of the prognosis in colorectal cancer patients.

https://mbrc.shirazu.ac.ir/article_3231_a7a392e08e513e577af96cfd9b8706b6.pdf

Shiraz University Press Molecular Biology Research Communications 2322-181X 4 4 2015 12 01 Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene 225 237 3265 10.22099/mbrc.2015.3265 EN Majidreza Khoshkholgh Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmehsara, Iran Sajad Nazari Genetic and Breeding Research Center for Coldwater Fishes, Yasouj, Iran Journal Article 2015 04 27 The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each in Anzali wetland and Aras reservoir and three rivers Chafrood, Masule Rudkhan and Siah Darvishan. The PCR products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (DdeІ, MboІ, TaqI, RsaІ and HinfІ). Twenty eight composite haplotypes were showed with the number of haplotypes in each population sample ranging from 8 to 13. Private haplotypes were found at very low frequencies. Two regional (Siah Darvishan River and Astara) groups were clearly recognized by cluster and molecular variance model (AMOVA) analyses (P<0.0001). Each of these groups revealed dominant haplotypes while these haplotypes play less important rule in population structures of the other geographic areas. Intrapopulation haplotype (h) and nucleotide (π) diversities were high for each locality, ranging h=0.7560±0.030 and π= 0.00334±0.00301, respectively. Results of this study discerned two genetically divergent populations of narrow-clawed crayfish including Siah Darvishan River and Astara. Thus, the population structure of the narrow-clawed crayfish, as inferred from mtDNA analysis, is constituted by genetically separate groups that nearly reflect their geographic distribution.

https://mbrc.shirazu.ac.ir/article_3265_a1ac3058a017448170346f7a3395ffb2.pdf