Assessment of genetic diversity among sunflower genotypes using microsatellite markers

Document Type : Original article

Authors

1 Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Ardabil (Moghan), Iran

2 Department of Crop Production and Breeding, Faculty of Agriculture, Ataturk University, Erzurum, Turkey

3 Seed and Plant Improvement Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding field crops. Twenty-one microsatellite markers used to assess genetic diversity and relationship of 68 sunflower genotypes (Helianthus annuus L.). All of 21 pairs of SSR (Simple Sequence Repeats) markers produced a total number of 49 polymorphic bands. DNA fragments ranged from 92 to 850 bp. The highest and lowest polymorphic information content (PIC) values were determined as 0.58 and 0.10 for marker Ha806-ar and Ha494-ar. The number of alleles per locus was calculated as 2-6 with the average of 2.86. In this study, CMS (Cytoplasmic Male Sterility) lines showed the highest and Iranian hybrids showed the least polymorphism, respectively. Principal coordinates analysis revealed that Iranian hybrids were well-separated compare to the other groups. The analysis of molecular variance (AMOVA) indicated higher genetic variation within groups (90%) rather than among groups (10%). This study revealed that the SSR markers such as Ha806-ar could be a useful tool for distinguishing sunflowers genotypes. According to the study, there is a significant genetic distance among individuals. Parental lines (R26 and CMS502 lines with lowest similarity coefficient) may be useful for future sunflower crossing and hybrid breeding programs. Generally, high similarity coefficient estimation among investigated sunflower groups revealed that there was a narrow genetic base in investigated materials suggesting broadening its genetic base by introduction of new genes into existing breeding materials.

Keywords

References

1. Hu J,  Seiler G,  Kole C.  Genetics, Genomics and Breeding of sunflower: CRC Press; 2010.
2. Zeinalzadeh Tabrizi H, Ghaffari M. Production of sunflower hybrids based on new cytoplasmic male sterility sources. 3rd International Symposium on Plant Protection and Plant Health in Europe; 2009; Berlin, Germany.
3. Liu A, Burke JM. Patterns of nucleotide diversity in wild and cultivated sunflower. Genetics 2005;173:321-330.
4. Sahranavard Azartamar F, Darvishzadeh R, Ghadimzadeh M, Azizi H, Aboulghasemi Z. Identification of SSR loci related to some important agro morphological traits in different oily sunflower (Helianthus annuus L.) lines using association mapping. J Plant Gene Res 2015;2:15-32. [In Persian]
5. Lawson W, Henry R, Kochman J, Kong G. Genetic diversity in sunflower (Helianthus annuus L.) as revealed by random amplified polymorphic DNA analysis. Crop Pasture Sci 1994;45:1319-1327.
6. Liu J, Liu G-S, Jan C-C. Comparison of genetic diversity of the germplasm resources of confectionary sunflower (Helianthus annuus) in China based on RAPDs and AFLPs. Acta Bot Sin 2003;45:352-358.
7. Yue B, Cai X, Vick BA, Hu J. Genetic diversity and relationships among 177 public sunflower inbred lines assessed by TRAP markers. Crop Sci 2009;49:1242-1249.
8. Gentzbittel L, Zhang Y-X, Vear F, Griveau B, Nicolas P. RFLP studies of genetic relationships among inbred lines of the cultivated sunflower, Helianthus annuus L.: evidence for distinct restorer and maintainer germplasm pools. Theor Appl Genet 1994;89:419-425.
9. Eryiğit T, Kumlay AM, Sancaktaroğlu S. Ayçiçeği (Helianthus annuus L.) çeşit teşhisinde morfolojik, biyokimyasal ve moleküler tekniklerin kullanımı. 9 Tarla Bitkileri Kongresi 2011; Bursa/Turkey. [In Turkish]
10. Zeinalzadeh-Tabrizi  H, Haliloglu K, Razban-Haghighi A. Genetic diversity of sunflower genotypes (Helianthus annuus L.) using TRAP markers. Crop Biotechnol 2015;12:39-53. [In Persian]
11. Jannatdoust M, Darvishzadeh R, Azizi H, Ebrahimi M, Ziaefard R, Gholinezhad E. Identification of retrotransposon markers associated with agromorphological traits in confectionary sunflower (Helianthus annuus L.) under normal and drought stress conditions. J Crop Breed 2017;8:183-197.
12. Hatami MH, Darvishzadeh R, Mohseni Z. Evaluation of genetic diversity and classification of advanced sunflower lines using ISSR markers. J Agr Biotechnol 2014;3:33-44. [In Persian]
13. Basirnia A, Darvishzadeh R, Mandoulakani BA. Retrotransposon insertional polymorphism in sunflower (Helianthus annuus L.) lines revealed by IRAP and REMAP markers. Plant Biosyst 2014;1-12.
14. ZeinalzadehTabrizi H. Genetic diversity of sunflower genotypes using SSR and TRAP markers. Ph.D. Thesis. Graduate School of Natural and Applied Sciences. Ataturk University, Erzurum, Turkey; 2014 [In Turkish]
15. ZeinalzadehTabrizi H, Hosseinpour A, Aydin M, Haliloglu K. A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes. J Biol Env Sci 2015; 7:222-225.
16. Tang S, Yu J-K, Slabaugh M, Shintani D, Knapp S. Simple sequence repeat map of the sunflower genome. Theoretical and Applied Genetics. 2002;105:1124-1136.
17. Paniego N, Echaide M, Muñoz M, Fernández L, Torales S, Faccio P, Fuxan I,  Carrera M,  Zandomeni R, Suárez, EY. Microsatellite isolation and characterization in sunflower (Helianthus annuus L.). Genome 2002;45:34-43.
18. Rohlf FJ. NTSYS-pc: numerical taxonomy and multivariate analysis system: Applied Biostatistics; 1992.
19. Yeh F, Yang R, Boyle T, Ye Z, Mao J. POPGEN Ver. 1.32. The user-friendly software for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Alberta, Canada. 1997.
20. Peakall R, Smouse PE. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 2006;6:288-295.
21. Liu K, Muse SV. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 2005;21:2128-2129.
22. Anderson JA, Churchill G, Autrique J, Tanksley S, Sorrells M. Optimizing parental selection for genetic linkage maps. Genome 1993;36:181-186.
23. Nei M, Tajima F, Tateno Y. Accuracy of estimated phylogenetic trees from molecular data. J Mol Evol 1983;19:153-170.
24. Lewontin RC. The Apportionment of Human Diversity. Evolutionary Biology: Springer; 1995.p.381-398.
25. Lochner TC, Agriculturae MS. Prediction of heterotic groups and hybrid performance in south african sunflower (Helianthus annuus L.) germplasm using SSR analysis. Msc Thesis, University of the Free State, South Africa. 2011.
26. Erasmus TE. Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis. Ph.D. Thesis, University of KwaZulu-Natal, Pietermaritzburg, South Africa. 2010.
27. Darvishzadeh R, Azizi M, Hatami-Maleki H, Bernousi I, Abdollahimandoulakani B, Jafari M, Sarrafi A. Molecular characterization and similarity relationships among sunflower (Helianthus annuus L.) inbred lines using some mapped simple sequence repeats. Afr J Biotechnol 2010;9:7280-7288.
28. Gholizadeh S, Darvishzadeh R, Mandoulakani BA, Bernousi I, Alavi SR, Masouleh AK. Molecular characterization and similarity relationships among flue-cured tobacco (Nicotiana tabacum L.) genotypes using simple sequence repeat markers. Not Bot Horti Agrobot Cluj Napoca 2012;40:247-253.
29. Antonova T, Guchetl S, Tchelustnikova T, Ramasanova S. Development of marker system for identification and certification of sunflower lines and hybrids on the basis of SSR-analysis. Helia 2006;29:63-72.
30. Solodenko A, Sivolap Y. Genotyping of Helianthus based on microsatellite sequences. Helia 2005;28:19-26.
31. Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 1973;70:3321-3323.
32. Shannon CE, Weaver W. The mathematical theory of communication (Urbana, IL. University of Illinois Press IL; 1949.
33. Dong G, Liu G, Li K. Studying genetic diversity in the core germplasm of confectionary sunflower (Helianthus annuus L.) in China based on AFLP and morphological analysis. Russ J Genet 2007;43:627-635.
34. Hongtrakul V, Huestis GM, Knapp SJ. Amplified fragment length polymorphisms as a tool for DNA fingerprinting sunflower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 1997;95:400-407.
35. Colombo C, Nucci S, Priolli R, Zucchi M, Carhalho C, Chrfl B. Genetic diversity of Macaw Palm (Acrocomia aculeata) by microsatellite markers. Proceedings of the International Plant and Animal Genome Conference, San Diego, CA, USA; 2013.
36. Jannatdoust M, Darvishzadeh R, Ziaeifard R, Ebrahimi MA, Maleki HH, Gholinezhad E. Analysis of genetic diversity and population structure of confectionery sunflower (Helianthus annuus L.) native to Iran. J Crop Sci Biotechnol 2016;19:37-44.
37. Solouki M, Mehdikhani H, Zeinali H, Emamjomeh A. Study of genetic diversity in Chamomile (Matricaria chamomilla) based on morphological traits and molecular markers. Sci Hortic 2008;117:281-287.
38. Kholghi M, Darvishzadeh R, Bernousi I, Pirzad A, Laurentin H. Assessment of genomic diversity among and within Iranian confectionery sunflower (Helianthus annuus L.) populations by using simple sequence repeat markers. Acta Agr Scand B Sil Plant Sci 2012;62:488-498.
39. Tang S, Knapp SJ. Microsatellites uncover extraordinary diversity in native American land races and wild populations of cultivated sunflower. Theor Appl Genet 2003; 106:990-1003.
40. Ronicke S, Hahn V, Friedt W. Resistance to Sclerotinia sclerotiorum of ‘high oleic’ sunflower inbred lines. Plant Breed 2005;124:376-381.

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