Association between GSTM1 and GSTT1 polymorphisms and susceptibility to methamphetamine dependence

Document Type : Original article

Authors

Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran

Abstract

Glutathione S-transferases (GSTs; EC: 2.5.1.18) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. Functional genetic polymorphisms in genes encoding GSTM1 (a member of GST class mu; OMIM: 138350), and GSTT1 (a member of GST class theta; OMIM: 600436) have been well defined. The functional null alleles of GSTM1 and GSTT1 represent deletions of GSTM1 and GSTT1 genes, respectively. The aim of the present study is to investigate the association between GSTM1 and GSTT1 polymorphisms and methamphetamine dependence. The present population-based case-control study was performed in Shiraz (southern Iran). In total, 52 methamphetamine dependence (11 females, 41 males) and 635 healthy controls (110 females, 525 males) were included in this study. The genotypes of GSTM1 and GSTT1 polymorphisms were determined by PCR. Neither GSTM1 (OR=0.92, 95% CI: 0.52-1.61, P=0.771) nor GSTT1 (OR=0.71, 95% CI: 0.33-1.54, P=0.381) null genotypes were significantly associated with risk of methamphetamine dependence. It should be noted that although there was no association between the GSTM1 null genotype and risk of methamphetamine dependence, in both genders, there was significant interaction between gender and GSTM1 polymorphism (P=0.029). The combination genotypes of the GSTM1 and GSTT1 polymorphisms revealed that the genotypes of these two polymorphisms had no additive effect in relation to the susceptibility to methamphetamine dependence. The present study revealed that genetic polymorphisms of GSTT1 and GSTM1 are not risk factors for methamphetamine dependence.

Keywords

References

1. Singleton J, Degenhardt L, Hall W, Zabransky T. Mortality among amphetamine users: A systematic review of cohort studies. Drug Alcohol Depend 2009;105:1-8.
2.  Gelernter J, Kranzler HR. Genetics of drug dependence. Dialogues Clin Neurosci 2010;12:77-84.
3. Solhi H, Malekirad A, Kazemifar AM, Sharifi F. Oxidative stress and lipid peroxidation in prolonged users of methamphetamine. Drug Metab Lett 2014;7:79-82.
4. Ghazavi A, Mosayebi G, Solhi H, Rafiei M, Moazzeni SM. Serum markers of inflammation and oxidative stress in chronic opium (Taryak) smokers. Immunol Lett 2013;153:22-26. 
5. Tata DA, Yamamoto BK. Interactions between methamphetamine and environmental stress: role of oxidative stress, glutamate and mitochondrial dysfunction. Addiction 2007;102 Suppl 1:49-60. 
6.  Brown JM, Yamamoto BK. Effects of amphetamines on mitochondrial function: role of free radicals and oxidative stress. Pharmacol Therapeut 2003;99:45-53.
7.  Castro AA, Moretti M, Casagrande TS, Martinello C, Petronilho F, Steckert AV, Guerrini R, Calo' G, Dal Pizzol F, Quevedo J, Gavioli EC. Neuropeptide S produces hyperlocomotion and prevents oxidative stress damage in the mouse brain: A comparative study with amphetamine and diazepam. Pharmacol Biochem Behav 2009;91:636-642.
8.   Shin EJ, Duong CX, Nguyen XKT, Li Z, Bing G, Bach JH, Park DH, Nakayama K, Ali SF, Kanthasamy AG, Cadet JL, Nabeshima T, Kim HC. Role of oxidative stress in methamphetamine-induced dopaminergic toxicity mediated by protein kinase. Behav Brain Res 2012;232:98-113.
9.  Perfeito R, Oliveira TC, Rego AC. Revisiting oxidative stress and mitochondrial dysfunction in the pathogenesis of Parkinson disease, resemblance to the effect of amphetamine drugs of abuse. Free Radic Biol Med 2012;53:1791-1806.
10.Harada S, Misawa S, Nakamura T, Tanaka N, Ueno E, Nozoe M. Detection of GST1 gene deletion by the polymerase chain reaction and its possible correlation with stomach cancer in Japanese. Hum Genet 1992;90:62-64.
11. Pemble S, Schroeder KR, Spencer SR, Meyer DJ, Hallier E, Bolt HM, Ketterer B, Taylor JB. Human glutathione S-transferase theta (GSTT1): cDNA cloning and the characterization of a genetic polymorphism. Biochem J 1994;300:271-276.
12. Kelada SN, Kardia SL, Walker AH, Wein AJ, Malkowicz SB, Rebbeck TR. The   glutathione S-transferase-mu and –theta genotypes in the etiology of prostate cancer: genotype-environmental interaction with smoking. Cancer Epidemiol Biomarkers Prev 2000;9:1329-1334.
13. Saadat I, Saadat M. The glutathione S-transferase mu polymorphism and susceptibility to acute lymphocytic leukemia. Cancer Lett 2000;158:43-45.
14. Saadat I, Saadat M. Glutathione S-transferase M1 and T1 null genotypes and the risk of gastric and colorectal cancers. Cancer Lett 2001;169:21-26.
15. Hashibe M, Brennan P, Strange RC, Bhisey R, Cascorbi I, Lazarus P, Oude Ophuis MB, Benhamou S, Foulkes WD, Katoh T, Coutelle C, Romkes M, Gaspari L, Taioli E, Boffetta P. Meta- and pooled analyses of GSTM1, GSTT1, GSTP1 and CYP1A1 genotypes and risk of head and neck cancer. Cancer Epidemiol Biomarkers Prev 2003;12:1509-1517.
16. Abbas A, Delvinquiere K, Lechevrel M, Lebailly P, Gauduchon P, Launoy G, Sichel F. GSTM1, GSTT1, GSTP1 and CYP1A1 genetic polymorphisms and susceptibility to esophageal cancer in a French population: Different pattern of squamous cell carcinoma and adenocarcinoma. World J Gastroenterol 2004;10: 3389-3393.
17. Saadat M, Mobayen F, Farrashbandi H. Genetic polymorphism of glutathione S-transferase T1: A candidate genetic modifier of individual susceptibility to schizophrenia. Psychiatry Res 2007;153:87-91.
18. Harada S, Tachikawa H, Kawanishi Y. Glutathione S-transfearse M1 gene deletion may be associated with susceptibility to certain forms of schizophrenia. Biochem Biophys Res Commun 2001;281:267-271.
19. Mohammadynejad P, Saadat I, Ghanizadeh A, Saadat M. Bipolar disorder and polymorphisms of glutathione S-transferases M1 (GSTM1) and T1 (GSTT1). Psychiatry Res 2011;186:144-146.
20. Saadat M, Saadat I, Saboori Z, Emad A. Combination of CC16, GSTM1 and GSTT1 polymorphisms is associated with asthma. J Allergy Clin Immunol 2004;113:996-998.       
21. Liang S, Wei X, Gong C, Wei J, Chen Z, Chen X, Wang Z, Deng J. Significant association between asthma risk and the GSTM1 and GSTT1 deletion polymorphisms: an updated meta-analysis of case-control studies. Respirology 2013;18:774-783.
22. Saadat M, Farvardin-Jahromi M, Saadat H. Null genotype of glutathione S-transferase M1 is associated with senile cataract susceptibility in non-smoker females. Biochem Biophys Res Commun 2004;319:1287-1291.
23. Saadat M, Farvardine-Jahromi M. Polymorphism of glutathione S-transferase M1, occupational exposure to sunlight, and senile cataract risk. Occup Environ Med 2006;63:503-504.
24. Wilson MH, Grant PJ, Hardie LJ, Wild CP. Glutathione S-transferase M1 null genotype is associated with a decreased risk of myocardial infraction. FASEB J 2000;14:791-796.
25. Juronen E, Tasa G, Uuskula M, Pooga M, Mikelsaar AV. Purification, characterization and tissue distribution of human class theta glutathione S-transferase T1-1. Biochem Mol Biol Int 1996;39:21-29. 
26. Listowsky I, Rowe JD, Patskovsky YV, Tchaikovskaya T, Shintani N, Novikova E, Nieves E. Human testicular glutathione S-transferases: insights into tissue-specific expression of the diverse subunit classes. Chemico-Biol Interact 1998;111-112:103-112.
27. Nakatome M, Miyaji A, Mochizuki K, Kishi Y, Isobe I, Matoba R. Association between the GST genetic polymorphisms and methamphetamine abusers in the Japanese population. Leg Med (Tokyo) 2009;11 Suppl 1:S468-470.
28. Koizumi H, Hashimoto K, Kumakiri C, Shimizu E, Sekine Y, Ozaki N, Inada T, Harano M, Komiyama T, Yamada M, Sora I, Ujike H, Takei N, Iyo M. Association between the glutathione S-transferase M1 gene deletion and female methamphetamine abusers. Am J Med Genet B Neuropsychiatr Genet 2004;126B: 43-45.
29. Rafiee L, Saadat I, Saadat M. Glutathione S-transferase genetic polymorphisms (GSTM1, GSTT1 and GSTO2) in three Iranian populations. Mole Biol Rep 2010;37: 155-158.
30. Saadat M, Saadat I. Prevalence of G6721T polymorphism of XRCC7 in an Iranian population. EXCLI Journal 2012;11:93-97.
31. Hayes JD, Strange RC. Glutathione S-transferase polymorphisms and their biological consequences. Pharmacology 2002;61:154-166.

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