Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)

Document Type : Original article

Authors

1 Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran.

2 Iran Fishery Research Organization (IFRO), Tehran, Iran.

3 International Sturgeon Research Institute, Rasht-Iran.

Abstract

The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon. 

Keywords

References

  1. Pourkazemi M. Caspian Sea sturgeon conservation and fisheries: Past, and Future. J Appl Ichthyol 2006; 22: 12-16.
  2. Williot P, Arlati G, Chebanov M, Gulyas T, Kasimov R, Kirschbaum F, Patriche N, Pavlovskaya LP, Poliakova L, Pourkazemi M, Kim Y, Zhuang P, Zholdasova I. Status and management of Eurasian sturgeon: an overview. Internat. Rev. Hydrobiol 2002; 87:483-506.
  3. Perez-Sanchez J, Smel J, Le Bail PY. Lacation and characterizeation of growth hormone binding sites in the central nervous system of a teleost fish (Oncorhynchus myksis). Growth Regul 1991; 1: 145-152.
  4. Sakamoto T, Hirano T. Growth Hormone receptors in the liver and osmoregulatory organs of rainbow trout: characterization and dynamics during adaptation to seawater. J Endocrinol 1991; 130: 425-433.

  5. Bjornsson BTH.The biology of salmon growth hormone: form daylight to dominance. Fish Physiol Biochem 1997; 17: 9-24.

  6. Bjornsson BTH, Johansson V, Benedet S, Einarsdottir IE, Hildahl J, Agustsson T, Jonsson E. Growth hormone endocrinology of salmonids: regulatory mechanisms and mode of action. Fish Physiol Biochem 2004; 17:9-242
  7. Perez-Sanchez J. The involvement of growth hormone in growth regulation, energy homeostasis and immunefunction in the Gilthead Sea bream (Sparus aurata). Fish Physiol Biochem 2000; 22:135-144.
  8. Peter RE, Marchant TA. The endocrinology of growth in carp and related species. Aquaculture 1995; 129: 299-321.
  9. Funkenstein B, Cohen I. Ontogeny of growth hormone protein and mRNA in the gilthead sea bream Sparus aurata. Growth Regul 1996; 6:16-21.
  10. Yang BY, Greene M, Chen TT. Early embryonic expression of the growth hormone family protein genes in the developing rainbow trout, Oncorhynchus myksis. Mol Reprod Dev1999; 53: 127-134.
  11. De Jesus EGT, Ayson FG, Amemiya Y, Hyodo S, Hirano T, Kawauchi H. Milk fish (chanos chanos) growth hormone cDNA cloning and mRNA expression in embryo and early larval stages .Aquaculture 2002; 208: 177-188.
  12. Li WS, Chen D,Wohg AO, Lin HR. Molecular cloning, tissue distribution, and ontogeny of mRNA expression of growth hormone in orange-spotted grouper (Epinephelus coioides). Gen Comp Endocrinol 2005; 144: 78-89.
  13. Revol A, Rodriguez MLG, Montenegro VH, Aguilera C, Saldana HB, Mendoza R. Cloning of the growth hormone cDNA of alligator gar Atractosteus spatula and its expression through larval development. Comp Biochem Physiol 2005; 140: 423-429.
  14. Ozaki Y, Fukada H, Tanaka H, Kagawa H, Ohta H, Adachi H, Akihiko H, Yamauchi K. Expression of Growth hormone family and growth hormone receptor during early development in the Japanese eel (Anguilla japonica). Comp Biochem Physiol 2006; 145: 27-34.
  15. Cao H, Zhou RJ, Wei QW, Li CG, Gui JF. Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages. J Appl Ichthyol 2011; 27: 501-504.
  16. Kolangi Miandare H, Farahmand H, Akbarzadeh A, Ramezanpour S, Kaiya H, Miyazato M, Rytkönen KT, Nikinmaa M. Developmental transcription of genes putatively associated with growth in two sturgeon species of different growth rate. Gen Comp Endocrinol 2013; 182:41–47.
  17. Pourdehghani M, kazemi R, Yazdani MA, Pourkazemi M, Vahabzade H, Nazari RM, Behmanesh SH. Investigation of dosages suitable hormone LHRH-A2 and optimal conditions on the artificial propagation of sturgeon breeding stocks cultured of native and non-native of Iran. Aquaculture: In proceedings of the 1st Iranian Aquaculture symposium. Iran, Rasht, Anzali 2011.pp. 430- 434.
  18. Chomczynski P, Sacchi N. Single-Step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction. Anal Biochem 1987; 162: 156-159.
  19. Akbarzade A, Farhmand H, Mahjoubi F, Nematollahi MA, Leskinen P, Rytkonen K, Nikinmaa M. The transcription of 1 - gulono -gamma - lactonoxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.Comp Biochem Physiol B Biochem Mol Biol 2011; 158: 282-288.
  20. Radonic A, Thulke S, Mackay I M, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real–time PCR. Biochem Biophys Res Commun 2004; 313:856-873.
  21. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-(ΔΔct) Method. Methods 2001; 25: 402-408.
  22. Golos TG, Durining M, Fisher JM, Fowler PD. Cloning of four growth hormone / chorionic somatomammo tropin-related complementary deoxyri- bonucleic acids differentially expressed during pregnancy in the rhesus monkey placenta. Endocrinology 1993; 133: 1744-1752.
  23. Lacroix MC, evinoy ED, Servely JL, Puissan C, Kann G. Expression of the growth hormone gene in ovine placenata: detection and cellular localization of the growth hormone gene in ovine placenta: detection and cellular localization of the protein. Endocrinology 1996; 137: 4886-4892.
  24. Frankenne F, Closset J, Gomez F, Scippo M L, Smal J, Nsnnen GH. The physiology of growth hormones (GHS) in pregnant women and partial characterization of the placental GH variant. J Clin Endocrinol Metab 1988; 66: 1171-1180.
  25. Ostaszewska T, Dabrowski T. Early development of Acipenseriformes (Chondrostei, Actinopterygii), in: Y. W. Kunz, C. A. Luer, B.G. Kapoor (Ed.).Development of Non-teleost Fishes. Science publishers 2009; USA, PP: 170-229.
  26. Gisbert E, Sarasquete M C, Wiolliot P, Castello-Orvay F. Histochemistery of the development of the digestive system of Siberian sturgeon during early ontogeny. J fish Biol 1999; 555: 96-616.
  27. Gisbert E. Early development and allometric growth patterns in Siberian sturgeon and their ecological significance. J Fish Biol 1999; 54: 825-826.
  28. Rodrlguez A, Gisbert E. Eye development and the role of vision during Siberian sturgeon early ontogeny. J Appl Ichthyol 2002; 18: 280-285.
  29. Gisbert E, Williot P, Castello-Orvay F. Influence of egg size on growth and survival of early stages of Siberian sturgeon (Acipenser baerii) under small scale hatchery conditions. Aquaculture 2000; 183: 83-94.
  30. Gisbert E, Rodrlguez A, CastelloOrvay F, Williot P. A histological study of the development of digestive tract of Siberian sturgeon (Acipenser baerii) during early ontogeny. Aquaculture 1998; 167: 195-209.
  31. Szcepkowski M, Kolman R, Szczepkowska B. Postembryonic Development, Survival and growth rate of Siberian sturgeon (Acipenser baeri Brandt) Larvae. Arch Ryb Pol 2000; 8: 193-204.

 

Molecular Biology Research Communications
Volume 4, Issue 4 - Serial Number 4
December 2015
Pages 181-188

Files

Share

How to cite

Statistics