Genetic polymorphism of N142D GSTO2 and susceptibility to breast cancer: a meta-analysis

Document Type : Original article


Shiraz University


To establish a comprehensive picture of the relationship between glutathione S-transferase omega 2 (GSTO2; MIM: 612314) gene N142D variant (rs. 156697) and breast cancer risk, the present meta-analysis was carried out. Studies published up to July 2012 with information about GSTO2 polymorphism and breast cancer risk were identified using several electronic databases. We identified 4 eligible studies, including 2678 subjects (1316 patients, and 1362 healthy controls) in relation to the N142D polymorphism of GSTO2 and risk of breast cancer. There was no heterogeneity between studies. Considering all of the studies, the DD (OR=1.29, 95%CI: 0.99-1.67, P=0.055) and ND (OR=1.03, 95%CI: 0.88-1.21, P=0.697) genotypes, did not alter the risk of breast cancer in comparison with the NN genotype. Therefore, it is suggested that if the number of studies increased, finding a significant association between N142D polymorphism of GSTO2 and susceptibility to breast cancer would be very probable.


1. Whitbread AK, Tetlow N, Eyre HJ, Sutherland GR, Board PG. Characterization of the human omega class glutathione transferase genes and associated polymorphisms. Pharmacogenetics 2003;13:131-144.
2. Mukherjee B, Salavaggione OE, Pelleymounter LL, Moon I, Eckloff BW, Schaid DJ, Wieben ED, Weinshilboum RM. Glutathione S-transferase omega 1 and omega 2 pharmacogenomics. Drug Metab Dispos 2006;34:1237-1246.
3. Masoudi M, Saadat M. Arsenic, GSTO2 Asp142Asp polymorphism, health and treatment. EXCLI Journal 2008;7:115-118.
4. Masoudi M, Saadat I, Omidvari S, Saadat M. Association between N142D genetic polymorphism of GSTO2 and susceptibility to colorectal cancer. Mol Biol Rep 2011;38:4309-4313.
5. Pongstaporn W, Pakakasama S, Sanguansin S, Hongeng S, Petmitr S. Polymorphism of glutathione S-transferase Omega gene: association with risk of childhood acute lymphoblastic leukemia. J Cancer Res Clin Oncol 2009;135:673-678.
6. Wang YH, Yeh SD, Shen KH, Shen CH, Juang GD, Hsu LI, Chiou HY, Chen CJ. A significantly joint effect between arsenic and occupational exposures and risk genotypes/diplotypes of CYP2E1, GSTO1 and GSTO2 on risk of urothelial carcinoma. Toxicol Appl Pharmacol 2009; 241:111-118.
7. Morari EC, Lima AB, Bufalo NE, Leite JL, Granja F, Ward LS. Role of glutathione-S-transferase and codon 72 of P53 genotypes in epithelial ovarian cancer patients. J Cancer Res Clin Oncol 2006;132:521-528.
8. Marahatta SB, Punyarit P, Bhudisawasdi V, Paupairoj A, Wongkham S, Petmitr S.  Polymorphism of glutathione S-transferase omega gene and risk of cancer. Cancer Lett 2006;236:276-281.
9. Chariyalertsak S, Purisa W, Sangrajrang S. Role of glutathione S-transferase omega gene polymorphisms in breast-cancer risk. Tumori 2009;95:739-743.
10. Masoudi M, Saadat I, Omidvari S, Saadat M. Additive effects of genetic variations of xenobiotic detoxification enzymes and DNA repair gene XRCC1 on the susceptibility to breast cancer. Breast Cancer Res Treat 2010;120:263-265.
11. Andonova IE, Justenhoven C, Winter S, Hamann U, Baisch C, Rabstein S, Spickenheuer A, Harth V, Pesch B, Brüning T, Ko YD, Ganev V, Brauch H. No evidence for glutathione S-transferases GSTA2, GSTM2, GSTO1, GSTO2, and GSTZ1 in breast cancer risk. Breast Cancer Res Treat 2010;121:497-502.
12. Richard F, Pacyna-Gengelbach M, Schlüns K, Fleige B, Winzer KJ, Szymas J, Dietel M, Petersen I, Schwendel A. Patterns of chromosomal imbalances in invasive breast cancer. Int J Cancer 2000;89:305-310.
13. Forozan F, Mahlamäki EH, Monni O, Chen Y, Veldman R, Jiang Y, Gooden GC, Ethier SP, Kallioniemi A, Kallioniemi OP. Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data. Cancer Res 2000;60: 4519-4525.
14. Jones C, Damiani S, Wells D, Chaggar R, Lakhani SR, Eusebi V. Molecular cytogenetic comparison of apocrine hyperplasia and apocrine carcinoma of the breast. Am J Pathol 2001; 158:207-214.
15. Dabbs DJ, Carter G, Fudge M, Peng Y, Swalsky P, Finkelstein S. Molecular alterations in columnar cell lesions of the breast. Mod Pathol 2006;19:344-349.
16. DerSimonian R, Laird N. Meta-analysis in clinical trials. Controlled Clin Trials 1986;7:177-188.
17. Mantel N, Haenszel W. Statistical aspects of the analysis of data from retrospective studies of disease. J Natl Cancer Inst 1959;22:719-748.
18. Masoudi M, Saadat I, Omidvari S, Saadat M. Additive effects of genetic variations of xenobiotic detoxification enzymes and DNA repair gene XRCC1 on the susceptibility to breast cancer. Breast Cancer Res Treat 2010;120:263-265.
19. Saadat M, Ansari-Lari M. Genetic polymorphism of glutathione S-transferase T1, M1 and asthma, a meta-analysis of the literature. Pak J Biol Sci 2007;10:4183-4189.
20. Saadat M. Apolipoprotein E (APOE) polymorphisms and susceptibility tobreast cancer: A meta-analysis. Cancer Res Treat 2012;44:121-126.
21. Saadat M. Genetic polymorphisms of glutathione S-transferase T1 (GSTT1) and susceptibility to gastric cancer: a meta-analysis. Cancer Sci 2006;97:505-509.
22. Kiyohara C, Takayama K, Nakanishi Y. Association of genetic polymorphisms in the base excision repair pathway with lung cancer risk: a meta-analysis. Lung Cancer 2006;54:267-283.
23. Hu Z, Ma H, Chen F, Wei Q, Shen H. XRCC1 polymorphisms and cancer risk: a meta-analysis of 38 case-control studies. Cancer Epidemiol Biomarkers Prev 2005;14:1810-1818.
24. Saadat I, Saadat M. Glutathione S-transferase M1 and T1 null genotypes and the risk of gastric and colorectal cancers. Cancer Lett 2001;169:21-26.
25. Saadat M, Saadat I, Saboori Z, Emad A. Combination of CC16, GSTM1, and GSTT1 genetic polymorphisms is associated with asthma. J Allergy Clin Immunol 2004;113:996-998.
26. Mohammadynejad P, Saadat I, Ghanizadeh A, Saadat M. Bipolar disorder and polymorphisms of glutathione S-transferases M1 (GSTM1) and T1 (GSTT1). Psychiatry Res 2011;186:144-146.